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<Structure load='4l3a' size='500' frame='true' align='right' caption='Internalin K bound to MVP' scene='Insert optional scene name here' />

== '''Introduction ==

'''Internalin K''' is a protein from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene]
, which is a [http://en.wikipedia.org/wiki/Gram-positive_bacteria Gram-positive bacterium] human pathogen. It has the ability to survive in the human intestine and to cross a variety of membranes, including mucosal, intestinal, placental, and blood–brain barriers, allows it to generate illnesses ranging from gastroenteritis in healthy individuals to bacteremia and meningitis in immunocompromised patients, as well as mother-to-child infections. [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene] can survive in a variety of cell types and proteins of the internalin family have been shown to play a key role in this survival.

'''Internalin K''' is involved in [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene] ability to escape from autophagy by recruitment of [http://en.wikipedia.org/wiki/Major_vault_protein major vault protein] to the bacterial surface.

=='''Structure'''==

===='''Internalin family's generalities'''====

[[Image:Internalin.jpg]]

Internalin family

----

'''L.monocytogenes''' uses a lot of virulence factors to initiate infection. Proteins of the '''internalin's family''', virulence factors, plays a key role in the infection's survival in a variety of cell types. They play key roles in processes ranging from adhesion to receptor recognition and are thus essential for infection. The internalin family uses a binding partner action.

'''The three-dimensional structure''' of the internalin family shows that there are modular proteins in order to improve the binding's partner. A common architecture is pointed, the N-terminal domain, also called N-terminal '''leucine-rich repeats''' (LRRs). It is composed of 22-residue regions including a β-strand and an helix. The structure is a curved solenoid. '''LRR''' is followed by domains in cell signaling and often in bacterial surface attachment. Whereas the C-terminal regions are not similar that contributes the variety of roles. Each internalin plays a specific role in the infection.

== '''Structure of Internalin K''' ==

[[Image:Chain1.png]]

'''Internalin K''' is a multi-domain virulence factor. It harbours four domains formed in the shape of '''"bent arm"'''.

Domains <scene name='56/568014/D1/1'>D1</scene> and <scene name='56/568014/D2/1'>D2</scene> are related to domains <scene name='56/568014/D3/1'>D3</scene> and <scene name='56/568014/D4/1'>D4</scene> by an 90° angle.

The <scene name='56/568014/D1/1'>D1</scene> domain composed of a two-helical cap region and also by the leucine-rich repeats (LRRs). It is stably associated to <scene name='56/568014/D2/1'>D2</scene>.

<scene name='56/568014/D2/1'>D2</scene> has few contacts with <scene name='56/568014/D3/1'>D3</scene> which improves the flexibility required by '''Internalin K''' to bind to its partner while remaining associated to the surface of the bacterium.

The association of <scene name='56/568014/D3/1'>D3</scene> and <scene name='56/568014/D2/1'>D2</scene> domains represent the "elbow" region. It means the recognition of major vault protein domain.

Domains <scene name='56/568014/D3/1'>D3</scene> and <scene name='56/568014/D4/1'>D4</scene>, located in tandem and related to each other by an approximate 2-fold axis, both fold into compact structures composed of three antiparallel strands packed against two small helices. There are [http://en.wikipedia.org/wiki/Immunoglobulin_superfamily immunoglobulin]-like domain.

<scene name='56/568014/D1/1'>D1</scene> and <scene name='56/568014/D2/1'>D2</scene> are involved in binding to protein partners while <scene name='56/568014/D3/1'>D3</scene> and <scene name='56/568014/D4/1'>D4</scene> most probably serve as pedestals. The flexibility between domains of its elongated structure may play a key role in this complex function.

===='''Function of internalin K'''====

<scene name='56/568014/D1/1'>D1</scene>

[http://en.wikipedia.org/wiki/Major_vault_protein major vault protein]

[http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene]

[http://en.wikipedia.org/wiki/Gram-positive_bacteria Gram-positive bacterium]

<scene name='56/568014/Major_vault_protein/1'>MVP</scene>

<scene name='56/568014/D2/1'>D2</scene>

<scene name='56/568014/D3/1'>D3</scene>

<scene name='56/568014/D4/1'>D4</scene>

File:Internalin.jpg

2014-01-07T19:20:50Z

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'''
== Introduction ==
'''

'''Internalin K''' is a protein from [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene]
, which is a Gram-positive bacterium human pathogen. Its ability to survive in the human intestine and to cross a variety of membranes, including mucosal, intestinal, placental, and blood–brain barriers, allows it to generate illnesses ranging from gastroenteritis in healthy individuals to bacteremia and meningitis in immunocompromised patients, as well as mother-to-child infections. [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene] can survive in a variety of cell types and proteins of the internalin family have been shown to play a key role in this survival.

'''The three-dimensional strsuctures of internalin protein''' have revealed a common N-terminal with leucine-rich repeats (LRRs). Their C-terminal is distinct and implies the specific role of the internalin. Among those proteins, '''Internalin K''', outcome of gene lmo1290 in [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene], is a surface-associated molecule that can interact with [http://en.wikipedia.org/wiki/Major_vault_protein major vault protein], a cytoplasmic rubonucleoprotein particle.

Internalin K is a four-domain protein with a “bent arm” morphology. The recognition of [http://en.wikipedia.org/wiki/Major_vault_protein major vault protein] domain is locatized at the “elbow” region.

'''Tania ...'''
ça c'est les liens pour

major vault protein : [http://en.wikipedia.org/wiki/Major_vault_protein major vault protein]

listeria monocytogene : [http://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogene]

ça peut toujours servir.
Noublie surtout pas de '''
== SAUVEGARDER AVANT DE FERMER LA PAGE :) ==
'''