Proteopedia - User contributions [en]

Sandbox Reserved 100

2017-03-04T21:32:05Z

Steven Russell:

===Monomers Per Turn===

The ARC-1 model has 6.4 monomer chains per turn (56.0 degrees rotation between monomers). This is more chains/turn than some previous type IV pilus models.

To visualize chains/turn, we show <scene name='69/699340/Arc-1_no_hydrogen/18'>just one atom per protein monomer chain</scene> (alpha carbon of Phe51). Then these chain-marking-atoms are <scene name='69/699340/Arc-1_no_hydrogen/20'>connected with with rods</scene>, and the resulting helix is viewed from one end. When counting the chains/turn, bear in mind that the first and last (to complete one turn) count as 1/2 chain each.

<table cellpadding="4" border="1">
<tr><td>
Pilus
</td><td>
Chains/Turn (Angle)
</td><td>
Image
</td></tr><tr><td>
''Geobacter sulfurreducens'' (type IVa, 61 amino acids, theoretical model, 2016)<ref name="xiao1" />
</td><td>
<span style="font-size:170%;">'''6.4''' (56.0°)</span>
</td><td>
[[Image:CPT-Gs-Xiao-end.png|175px]]
</td></tr><tr><td>
''Pseudomonas aeruginosa'' (type IVa, 150 amino acids, fiber diffraction, 2004)<ref>PMID: 15100690</ref>
</td><td>
<span style="font-size:170%;">'''4.0''' (90°)</span>
</td><td>
[[Image:CPT-Pa-end.png|175px]]
</td></tr><tr><td>
''Vibrio cholerae'' (type '''IVb''', 198 amino acids, cryo-EM, 2012)<ref>PMID: 22361030</ref>
</td><td>
<span style="font-size:170%;">'''3.7''' (96.8°)</span>
</td><td>
[[Image:CPT-Vc2012-end.png|175px]]
</td></tr><tr><td>
''Neisseria gonorrhoeae'' (type IVa, 165 amino acids, cryo-EM, 2006)<ref>PMID: 16949362</ref>
</td><td>
<span style="font-size:170%;">'''3.6''' (100.8°)</span>
</td><td>
[[Image:CPT-Ng-2hil-end.png|175px]]
</td></tr></table>

==Notes & References==
<references />

Sandbox Reserved 100

2017-03-04T21:31:29Z

Steven Russell:

===Monomers Per Turn===

[https://skfb.ly/66CXY link title]

The ARC-1 model has 6.4 monomer chains per turn (56.0 degrees rotation between monomers). This is more chains/turn than some previous type IV pilus models.

To visualize chains/turn, we show <scene name='69/699340/Arc-1_no_hydrogen/18'>just one atom per protein monomer chain</scene> (alpha carbon of Phe51). Then these chain-marking-atoms are <scene name='69/699340/Arc-1_no_hydrogen/20'>connected with with rods</scene>, and the resulting helix is viewed from one end. When counting the chains/turn, bear in mind that the first and last (to complete one turn) count as 1/2 chain each.

<table cellpadding="4" border="1">
<tr><td>
Pilus
</td><td>
Chains/Turn (Angle)
</td><td>
Image
</td></tr><tr><td>
''Geobacter sulfurreducens'' (type IVa, 61 amino acids, theoretical model, 2016)<ref name="xiao1" />
</td><td>
<span style="font-size:170%;">'''6.4''' (56.0°)</span>
</td><td>
[[Image:CPT-Gs-Xiao-end.png|175px]]
</td></tr><tr><td>
''Pseudomonas aeruginosa'' (type IVa, 150 amino acids, fiber diffraction, 2004)<ref>PMID: 15100690</ref>
</td><td>
<span style="font-size:170%;">'''4.0''' (90°)</span>
</td><td>
[[Image:CPT-Pa-end.png|175px]]
</td></tr><tr><td>
''Vibrio cholerae'' (type '''IVb''', 198 amino acids, cryo-EM, 2012)<ref>PMID: 22361030</ref>
</td><td>
<span style="font-size:170%;">'''3.7''' (96.8°)</span>
</td><td>
[[Image:CPT-Vc2012-end.png|175px]]
</td></tr><tr><td>
''Neisseria gonorrhoeae'' (type IVa, 165 amino acids, cryo-EM, 2006)<ref>PMID: 16949362</ref>
</td><td>
<span style="font-size:170%;">'''3.6''' (100.8°)</span>
</td><td>
[[Image:CPT-Ng-2hil-end.png|175px]]
</td></tr></table>

==Notes & References==
<references />

Sandbox Reserved 100

2017-03-04T21:29:06Z

Steven Russell:

===Monomers Per Turn===

<iframe src="https://sketchfab.com/models/c527acc9bdeb46179a0f0322f63a9b70/embed?camera=0" width="640" height="480" frameborder="0" allowfullscreen="allowfullscreen"></iframe>

The ARC-1 model has 6.4 monomer chains per turn (56.0 degrees rotation between monomers). This is more chains/turn than some previous type IV pilus models.

To visualize chains/turn, we show <scene name='69/699340/Arc-1_no_hydrogen/18'>just one atom per protein monomer chain</scene> (alpha carbon of Phe51). Then these chain-marking-atoms are <scene name='69/699340/Arc-1_no_hydrogen/20'>connected with with rods</scene>, and the resulting helix is viewed from one end. When counting the chains/turn, bear in mind that the first and last (to complete one turn) count as 1/2 chain each.

<table cellpadding="4" border="1">
<tr><td>
Pilus
</td><td>
Chains/Turn (Angle)
</td><td>
Image
</td></tr><tr><td>
''Geobacter sulfurreducens'' (type IVa, 61 amino acids, theoretical model, 2016)<ref name="xiao1" />
</td><td>
<span style="font-size:170%;">'''6.4''' (56.0°)</span>
</td><td>
[[Image:CPT-Gs-Xiao-end.png|175px]]
</td></tr><tr><td>
''Pseudomonas aeruginosa'' (type IVa, 150 amino acids, fiber diffraction, 2004)<ref>PMID: 15100690</ref>
</td><td>
<span style="font-size:170%;">'''4.0''' (90°)</span>
</td><td>
[[Image:CPT-Pa-end.png|175px]]
</td></tr><tr><td>
''Vibrio cholerae'' (type '''IVb''', 198 amino acids, cryo-EM, 2012)<ref>PMID: 22361030</ref>
</td><td>
<span style="font-size:170%;">'''3.7''' (96.8°)</span>
</td><td>
[[Image:CPT-Vc2012-end.png|175px]]
</td></tr><tr><td>
''Neisseria gonorrhoeae'' (type IVa, 165 amino acids, cryo-EM, 2006)<ref>PMID: 16949362</ref>
</td><td>
<span style="font-size:170%;">'''3.6''' (100.8°)</span>
</td><td>
[[Image:CPT-Ng-2hil-end.png|175px]]
</td></tr></table>

==Notes & References==
<references />

Sandbox Reserved 100

2017-03-04T21:15:53Z

Steven Russell:

===Monomers Per Turn===

<div class="sketchfab-embed-wrapper"><iframe width="640" height="480" src="https://sketchfab.com/models/f50ee70436064607940be49a356990e6/embed" frameborder="0" allowvr allowfullscreen mozallowfullscreen="true" webkitallowfullscreen="true" onmousewheel=""></iframe>

The ARC-1 model has 6.4 monomer chains per turn (56.0 degrees rotation between monomers). This is more chains/turn than some previous type IV pilus models.

To visualize chains/turn, we show <scene name='69/699340/Arc-1_no_hydrogen/18'>just one atom per protein monomer chain</scene> (alpha carbon of Phe51). Then these chain-marking-atoms are <scene name='69/699340/Arc-1_no_hydrogen/20'>connected with with rods</scene>, and the resulting helix is viewed from one end. When counting the chains/turn, bear in mind that the first and last (to complete one turn) count as 1/2 chain each.

<table cellpadding="4" border="1">
<tr><td>
Pilus
</td><td>
Chains/Turn (Angle)
</td><td>
Image
</td></tr><tr><td>
''Geobacter sulfurreducens'' (type IVa, 61 amino acids, theoretical model, 2016)<ref name="xiao1" />
</td><td>
<span style="font-size:170%;">'''6.4''' (56.0°)</span>
</td><td>
[[Image:CPT-Gs-Xiao-end.png|175px]]
</td></tr><tr><td>
''Pseudomonas aeruginosa'' (type IVa, 150 amino acids, fiber diffraction, 2004)<ref>PMID: 15100690</ref>
</td><td>
<span style="font-size:170%;">'''4.0''' (90°)</span>
</td><td>
[[Image:CPT-Pa-end.png|175px]]
</td></tr><tr><td>
''Vibrio cholerae'' (type '''IVb''', 198 amino acids, cryo-EM, 2012)<ref>PMID: 22361030</ref>
</td><td>
<span style="font-size:170%;">'''3.7''' (96.8°)</span>
</td><td>
[[Image:CPT-Vc2012-end.png|175px]]
</td></tr><tr><td>
''Neisseria gonorrhoeae'' (type IVa, 165 amino acids, cryo-EM, 2006)<ref>PMID: 16949362</ref>
</td><td>
<span style="font-size:170%;">'''3.6''' (100.8°)</span>
</td><td>
[[Image:CPT-Ng-2hil-end.png|175px]]
</td></tr></table>

==Notes & References==
<references />