3mij

Crystal structure of a telomeric RNA G-quadruplex complexed with an acridine-based ligand.

spinqualitylabelsall models 3mij, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3mij is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Ligands: K, R14 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Human telomeric DNA is now known to be transcribed into noncoding RNA sequences, termed TERRA. These sequences, which are believed to play roles in the regulation of telomere function, can form higher-order quadruplex structures and may themselves be the target of therapeutic intervention. The crystal structure of a TERRA quadruplex-acridine small-molecule complex at a resolution of 2.60 [Formula: see text], is reported here and contrasts remarkably with the structure of the analogous DNA quadruplex complex. The bimolecular RNA complex has a parallel-stranded topology with propeller-like UUA loops. These loops are held in particular conformations by multiple hydrogen bonds involving the O2' hydroxyl groups of the ribonucleotide sugars and play an active role in binding the acridine molecules to the RNA quadruplex. By contrast, the analogous DNA quadruplex complex has simpler 1:1 acridine binding, with no loop involvement. There are significant loop conformational changes in the RNA quadruplex compared to the native TERRA quadruplex ( Collie , G. W. ; Haider , S. M. ; Neidle , S. ; Parkinson , G. N. Nucleic Acids Res. 2010 , 38, , 5569 - 5580 ), which have implications for the future design of small molecules targeting TERRA quadruplexes, and RNA quadruplexes more generally. Structural Basis of Telomeric RNA Quadruplex-Acridine Ligand Recognition.,Collie GW, Sparapani S, Parkinson GN, Neidle S J Am Chem Soc. 2011 Feb 3. PMID:21291211 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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