4hm0 | pdb_00004hm0

Naphthalene 1,2-Dioxygenase bound to indole-3-acetate

spinqualitylabelsall models 4hm0, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4hm0 is a 2 chain structure with sequence from Pseudomonas sp. C18. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: EDO, FE, FES, IAC, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NDOB_PSEU8 Component of the naphthalene dioxygenase (NDO) multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into naphthalene to form cis-naphthalene dihydrodiol. Publication Abstract from PubMed Rieske nonheme iron oxygenases (ROs) are a well studied class of enzymes. Naphthalene 1,2-dioxygenase (NDO) is used as a model to study ROs. Previous work has shown how side-on binding of oxygen to the mononuclear iron provides this enzyme with the ability to catalyze stereospecific and regiospecific cis-dihydroxylation reactions. It has been well documented that ROs catalyze a variety of other reactions, including mono-oxygenation, desaturation, O- and N-dealkylation, sulfoxidation etc. NDO itself catalyzes a variety of these reactions. Structures of NDO in complex with a number of different substrates show that the orientation of the substrate in the active site controls not only the regiospecificity and stereospecificity, but also the type of reaction catalyzed. It is proposed that the mononuclear iron-activated dioxygen attacks the atoms of the substrate that are most proximal to it. The promiscuity of delivering two products (apparently by two different reactions) from the same substrate can be explained by the possible binding of the substrate in slightly different orientations aided by the observed flexibility of residues in the binding pocket. One enzyme, many reactions: structural basis for the various reactions catalyzed by naphthalene 1,2-dioxygenase.,Ferraro DJ, Okerlund A, Brown E, Ramaswamy S IUCrJ. 2017 Aug 8;4(Pt 5):648-656. doi: 10.1107/S2052252517008223. eCollection , 2017 Sep 1. PMID:28989720 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Dioxygenase 3D structures

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