4yir | pdb_00004yir

Crystal structure of Rad4-Rad23 crosslinked to an undamaged DNA

spinqualitylabelsall models 4yir, resolution 3.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4yir is a 4 chain structure with sequence from Saccharomyces cerevisiae S288C and Synthetic construct. This structure supersedes the now removed PDB entry 4u29. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.0501Å Ligands: G47 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RAD4_YEAST Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Publication Abstract from PubMed The xeroderma pigmentosum C (XPC) complex initiates nucleotide excision repair by recognizing DNA lesions before recruiting downstream factors. How XPC detects structurally diverse lesions embedded within normal DNA is unknown. Here we present a crystal structure that captures the yeast XPC orthologue (Rad4) on a single register of undamaged DNA. The structure shows that a disulphide-tethered Rad4 flips out normal nucleotides and adopts a conformation similar to that seen with damaged DNA. Contrary to many DNA repair enzymes that can directly reject non-target sites as structural misfits, our results suggest that Rad4/XPC uses a kinetic gating mechanism whereby lesion selectivity arises from the kinetic competition between DNA opening and the residence time of Rad4/XPC per site. This mechanism is further supported by measurements of Rad4-induced lesion-opening times using temperature-jump perturbation spectroscopy. Kinetic gating may be a general mechanism used by site-specific DNA-binding proteins to minimize time-consuming interrogations of non-target sites. Kinetic gating mechanism of DNA damage recognition by Rad4/XPC.,Chen X, Velmurugu Y, Zheng G, Park B, Shim Y, Kim Y, Liu L, Van Houten B, He C, Ansari A, Min JH Nat Commun. 2015 Jan 6;6:5849. doi: 10.1038/ncomms6849. PMID:25562780 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA repair protein Rad4 UV excision repair protein

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