6frh | pdb_00006frh
Crystal structure of Ssp DnaB Mini-Intein variant M86
Structural highlights
Function[DNAB_SYNY3] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity (By similarity). Publication Abstract from PubMedInteins remove themselves from a precursor protein by protein splicing. Due to the concomitant structural changes of the host protein, this self-processing reaction has enabled many applications in protein biotechnology and chemical biology. We show that the evolved M86 mutant of the Ssp DnaB intein displays a significantly improved tolerance towards non-native amino acids at the N-terminally flanking (-1) extein position compared to the parent intein, in the form of both an artificially trans-splicing split intein and the cis-splicing mini-intein. Surprisingly, side chains with increased steric bulk compared to the native Gly(-1) residue, including d-amino acids, were found to compensate for the essential block B histidine in His73Ala mutants in the initial N-S acyl shift of the protein splicing pathway. In the case of the M86 intein, large (-1) side chains can even rescue protein splicing activity as a whole. With the comparison of three crystal structures, namely of the M86 intein as well as of its Gly(-1)Phe and Gly(-1)Phe/His73Ala mutants, our data supports a model in which the intein's active site can exert a strain by varying mechanisms on the different angles of the scissile bond at the extein-intein junction to effect a ground-state destabilization. The compensatory mechanism of the block B histidine is the first example for the direct functional role of an extein residue in protein splicing. It sheds new light on the extein-intein interplay and on possible consequences of their co-evolution as well as on the laboratory engineering of improved inteins. A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.,Friedel K, Popp MA, Matern JCJ, Gazdag EM, Thiel IV, Volkmann G, Blankenfeldt W, Mootz HD Chem Sci. 2018 Oct 3;10(1):239-251. doi: 10.1039/c8sc01074a. eCollection 2019 Jan, 7. PMID:30713635[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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