7f8k | pdb_00007f8k

Room temperature structure of bacterial copper amine oxidase determined by serial femtosecond crystallography

spinqualitylabelsall models 7f8k, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7f8k is a 1 chain structure with sequence from "achromobacter_globiformis"_(conn_1928)_bergey_et_al._1930 "achromobacter globiformis" (conn 1928) bergey et al. 1930. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CU NonStd Res: TPQ Activity: Primary-amine oxidase, with EC number 1.4.3.21 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Recent advances in serial femtosecond X-ray crystallography (SFX) using X-ray free-electron lasers have paved the way for determining radiation-damage-free protein structures under nonfreezing conditions. However, the large-scale preparation of high-quality microcrystals of uniform size is a prerequisite for SFX, and this has been a barrier to its widespread application. Here, a convenient method for preparing high-quality microcrystals of a bacterial quinoprotein enzyme, copper amine oxidase from Arthrobacter globiformis, is reported. The method consists of the mechanical crushing of large crystals (5-15 mm(3)), seeding the crushed crystals into the enzyme solution and standing for 1 h at an ambient temperature of approximately 26 degrees C, leading to the rapid formation of microcrystals with a uniform size of 3-5 microm. The microcrystals diffracted X-rays to a resolution beyond 2.0 A in SFX measurements at the SPring-8 Angstrom Compact Free Electron Laser facility. The damage-free structure determined at 2.2 A resolution was essentially identical to that determined previously by cryogenic crystallography using synchrotron X-ray radiation. Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase.,Murakawa T, Suzuki M, Arima T, Sugahara M, Tanaka T, Tanaka R, Iwata S, Nango E, Tono K, Hayashi H, Fukui K, Yano T, Tanizawa K, Okajima T Acta Crystallogr F Struct Biol Commun. 2021 Oct 1;77(Pt 10):356-363. doi:, 10.1107/S2053230X21008967. Epub 2021 Sep 21. PMID:34605440 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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