7zm7 | pdb_00007zm7

CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM)

spinqualitylabelsall models 7zm7, resolution 2.77Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7zm7 is a 10 chain structure with sequence from Chaetomium thermophilum var. thermophilum DSM 1495. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.77Å Ligands: 2MR, 3PE, CDL, FES, FMN, LMT, NDP, PC1, ZMP, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function G1DJA6_CHATD Publication Abstract from PubMed Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic fungus Chaetomium thermophilum, determined by cryoEM up to 2.4-A resolution. We show that the complex undergoes a transition between two conformations, which we refer to as state 1 and state 2. The conformational switch is manifest in a twisting movement of the peripheral arm relative to the membrane arm, but most notably in substantial rearrangements of the Q-binding cavity and the E-channel, resulting in a continuous aqueous passage from the E-channel to subunit ND5 at the far end of the membrane arm. The conformational changes in the complex interior resemble those reported for mammalian complex I, suggesting a highly conserved, universal mechanism of coupling electron transport to proton pumping. Conformational changes in mitochondrial complex I of the thermophilic eukaryote Chaetomium thermophilum.,Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W Sci Adv. 2022 Nov 25;8(47):eadc9952. doi: 10.1126/sciadv.adc9952. Epub 2022 Nov , 25. PMID:36427319 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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