8ruw | pdb_00008ruw

Crystal structure of Archaeoglobus fulgidus (S)-3-O-geranylgeranylglyceryl phosphate synthase

spinqualitylabelsall models 8ruw, resolution 2.48Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ruw is a 6 chain structure with sequence from Archaeoglobus fulgidus DSM 4304. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.48Å Ligands: PO4, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GGGPS_ARCFU Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids. To a much lesser extent, is also able to use heptaprenyl pyrophosphate (HepPP; 35 carbon atoms) as the prenyl donor. Publication Abstract from PubMed Although ethers are common in secondary natural products, they are an underrepresented functional group in primary metabolism. As such, there are comparably few enzymes capable of constructing ether bonds in a general fashion. However, such enzymes are highly sought after for synthetic applications as they typically operate with higher regioselectivity and under milder conditions than traditional organochemical approaches. To expand the repertoire of well characterized ether synthases, we herein report on a promiscuous archaeal prenyltransferase from the scarcely researched family of geranylgeranylglyceryl phosphate synthases (GGGPSs or G(3)PSs). We show that the ultrastable Archaeoglobus fulgidus G(3)PS makes various (E)- and (Z)-configured prenyl glycerol ethers from the corresponding pyrophosphates while exerting perfect control over the configuration at the glycerol unit. Based on experimental and computational data, we propose a mechanism for this enzyme which involves an intermediary prenyl carbocation equivalent. As such, this study provides the fundamental understanding and methods to introduce G(3)PSs into the biocatalytic alkylation toolbox. Biocatalytic Ether Lipid Synthesis by an Archaeal Glycerolprenylase.,Kaspar F, Eilert L, Staar S, Oung SW, Wolter M, Ganskow CSG, Kemper S, Klahn P, Jacob CR, Blankenfeldt W, Schallmey A Angew Chem Int Ed Engl. 2024 Oct 2:e202412597. doi: 10.1002/anie.202412597. PMID:39359010 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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