9ezy

Vibrio cholerae DdmD-DdmE holo complex

spinqualitylabelsall models 9ezy, resolution 2.56Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9ezy is a 5 chain structure with sequence from Vibrio cholerae and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.56Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9KR73_VIBCH Publication Abstract from PubMed Seventh-pandemic Vibrio cholerae strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. In this study, we used cryogenic electron microscopy to determine the mechanistic basis for plasmid defense by DdmDE. The helicase-nuclease DdmD adopts an autoinhibited dimeric architecture. The prokaryotic Argonaute protein DdmE uses a DNA guide to target plasmid DNA. The structure of the DdmDE complex, validated by in vivo mutational studies, shows that DNA binding by DdmE triggers disassembly of the DdmD dimer and loading of monomeric DdmD onto the nontarget DNA strand. In vitro studies indicate that DdmD translocates in the 5'-to-3' direction, while partially degrading the plasmid DNA. These findings provide critical insights into the mechanism of DdmDE systems in plasmid elimination. Molecular mechanism of plasmid elimination by the DdmDE defense system.,Loeff L, Adams DW, Chanez C, Stutzmann S, Righi L, Blokesch M, Jinek M Science. 2024 Jul 12;385(6705):188-194. doi: 10.1126/science.adq0534. Epub 2024 , Jun 13. PMID:38870273 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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