Reticuline oxidase

spinqualitylabelsall models Structure of glycosylated California poppy reticuline oxidase complex with reticuline and FAD (PDB code 3d2d) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Reticuline oxidase or berberine bridge-forming enzyme (RO) is part of the alkaloids biosynthesis pathway catalyzing the conversion of (S)-reticuline to (S)-scoulerine. RO catalyzes the cyclization of the N-methyl moiety of (S)-reticuline into the berberine bridge carbon, C-8 of (S)- . Relevance Reticuline is a precursor of morphine and has potent central nervous system depressing effects. Structural highlights The 3D structure of the complex between RO and its substrate reticuline show the cofactor FAD having bicovalent attachment to RO His and Cys. FAD binding site. Water molecules are shown as red spheres. The substrate reticuline is seen in the active site cavity interacting with FAD and numerous RO sidechains. Whole active site. Active site cavity.

3D Structures of Reticuline oxidase

Updated on 02-February-2021

3d2h, 3d2j – CpRO + FAD – California poppy
3fw7, 3fw8, 4pzf – CpRO (mutant) + FAD
3d2d – CpRO + FAD + reticuline
3fwa, 4ec3 – CpRO (mutant) + FAD + reticuline
3fw9, 3gsy – CpRO + FAD + scoulerine derivative
3d2h – CpRO + FAD + ADP derivative

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