View source for 1yav


==Crystal structure of CBS domain-containing protein ykuL from Bacillus subtilis==
<StructureSection load='1yav' size='340' side='right'caption='[[1yav]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1yav]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YAV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yav OCA], [https://pdbe.org/1yav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yav RCSB], [https://www.ebi.ac.uk/pdbsum/1yav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yav ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1yav TOPSAN]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DARB_BACSU DARB_BACSU] Involved in the c-di-AMP-dependent regulation of the bacterial stringent response (PubMed:33619274, PubMed:35130724). Modulates the activities of at least two enzymes under conditions of potassium limitation (PubMed:33619274, PubMed:35130724). Apo-DarB regulates the activity of the GTP pyrophosphokinase RelA by interacting directly with RelA, leading to stimulation of (p)ppGpp synthesis and induction of the stringent response (PubMed:33619274). Apo-DarB also regulates pyruvate carboxylase (PYC) at two levels: directly at the protein level by binding to the enzyme and stimulating the synthesis of oxaloacetate and indirectly, by interaction with RelA, which leads to activation of the stringent response and to the increased expression of the pycA gene (PubMed:35130724). Stimulation of these enzymes by DarB is prevented in the presence of cyclic di-AMP (c-di-AMP) (PubMed:33619274, PubMed:35130724).<ref>PMID:33619274</ref> <ref>PMID:35130724</ref> 
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ya/1yav_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yav ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Burley SK]]
[[Category: Kumaran D]]
[[Category: Swaminathan S]]