View source for 4osp


==The crystal structure of urdamycin C-6 ketoreductase domain UrdMred with bound NADP and rabelomycin==
<StructureSection load='4osp' size='340' side='right'caption='[[4osp]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4osp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_fradiae Streptomyces fradiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OSP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2V4:RABELOMYCIN'>2V4</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4osp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4osp OCA], [https://pdbe.org/4osp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4osp RCSB], [https://www.ebi.ac.uk/pdbsum/4osp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4osp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K0IB23_STRFR K0IB23_STRFR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Angucyclines are tetracyclic polyketides produced by Streptomyces bacteria that exhibit notable biological activities. The great diversity of angucyclinones is generated in tailoring reactions, which modify the common benz[a]anthraquinone carbon skeleton. In particular, the opposite stereochemistry of landomycins and urdamycins/gaudimycins at C-6 is generated by the short-chain alcohol dehydrogenases/reductases LanV and UrdMred/CabV, respectively. Here we present crystal structures of LanV and UrdMred in complex with NADP+ and the product analog rabelomycin, which enabled us to identify four regions associated with the functional differentiation. The structural analysis was confirmed in chimeragenesis experiments focusing on these regions adjacent to the active site cavity, which led to reversal of the activities of LanV and CabV. The results surprisingly indicated that the conformation of the substrate and the stereochemical outcome of 6-ketoreduction appear to be intimately linked.

Structure-Based Engineering of Angucyclinone 6-Ketoreductases.,Patrikainen P, Niiranen L, Thapa K, Paananen P, Tahtinen P, Mantsala P, Niemi J, Metsa-Ketela M Chem Biol. 2014 Sep 3. pii: S1074-5521(14)00249-X. doi:, 10.1016/j.chembiol.2014.07.017. PMID:25200607<ref>PMID:25200607</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4osp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces fradiae]]
[[Category: Metsa-Ketela M]]
[[Category: Niiranen L]]
[[Category: Paananen P]]
[[Category: Patrikainen P]]