View source for 4uni


==beta-(1,6)-galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 in complex with galactose==
<StructureSection load='4uni' size='340' side='right'caption='[[4uni]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uni]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bifidobacterium_animalis_subsp._lactis_Bl-04 Bifidobacterium animalis subsp. lactis Bl-04]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UNI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uni OCA], [https://pdbe.org/4uni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uni RCSB], [https://www.ebi.ac.uk/pdbsum/4uni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uni ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Bifidobacterium genus harbours several health promoting members of the gut microbiota. Bifidobacteria display metabolic specialization by preferentially utilizing dietary or host derived beta-galactosides. This study investigates the biochemistry and structure of a glycoside hydrolase family 42 (GH42) beta-galactosidase from the probiotic Bifidobacterium animalis subsp. lactis Bl-04 (BlGal42A). BlGal42A displays a preference for undecorated beta1-6 and beta1-3 linked galactosides and populates a phylogenetic cluster with close bifidobacterial homologues implicated in the utilization of N-acetyl substituted beta1-3 galactosides from human milk and mucin. A long loop containing an invariant tryptophan in GH42, proposed to bind substrate at subsite +1, is identified here as specificity signature within this clade of bifidobacterial enzymes. Galactose binding at the subsite -1 of the active site induced conformational changes resulting in an extra polar interaction and the ordering of a flexible loop that narrows the active site. The amino-acid sequence of this loop provides an additional specificity signature within this GH42 clade. The phylogenetic relatedness of enzymes targeting beta1-6 and beta1-3 galactosides likely reflects structural differences between these substrates and beta1-4 galactosides, containing an axial galactosidic bond. These data advance our molecular understanding of the evolution of sub-specificities that support metabolic specialization in the gut niche.

A beta1-6/beta1-3 galactosidase from Bifidobacterium animalis subsp. lactis Bl-04 gives insight into sub-specificities of beta-galactoside catabolism within Bifidobacterium.,Viborg AH, Fredslund F, Katayama T, Nielsen SK, Svensson B, Kitaoka M, Leggio LL, Abou Hachem M Mol Microbiol. 2014 Oct 7. doi: 10.1111/mmi.12815. PMID:25287704<ref>PMID:25287704</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uni" style="background-color:#fffaf0;"></div>

==See Also==
*[[Galactosidase 3D structures|Galactosidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bifidobacterium animalis subsp. lactis Bl-04]]
[[Category: Large Structures]]
[[Category: Abou Hachem M]]
[[Category: Fredslund F]]
[[Category: Katayama T]]
[[Category: Kitaoka M]]
[[Category: Lo Leggio L]]
[[Category: Nielsen SK]]
[[Category: Svensson B]]
[[Category: Viborg AH]]