View source for 5wk4


==Crystal structure of an anti-idiotype VLR==
<StructureSection load='5wk4' size='340' side='right'caption='[[5wk4]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5wk4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Petromyzon_marinus Petromyzon marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WK4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.498&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wk4 OCA], [https://pdbe.org/5wk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wk4 RCSB], [https://www.ebi.ac.uk/pdbsum/5wk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wk4 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Variable lymphocyte receptors (VLRs), the leucine-rich repeat (LRR)-based antigen receptors of jawless fish, have great utility in a wide variety of biochemical and biological applications, similar to classical Ig-based antibodies. VLR-based reagents may be particularly useful when traditional antibodies are not available. An anti-idiotype lamprey VLR, VLR39, has previously been identified that recognizes the heavy-chain CDR3 of the B-cell receptor (BCR) of a leukemic clone from a patient with chronic lymphocytic leukemia (CLL). VLR39 was used successfully to track the re-emergence of this clone in the patient following chemotherapy. Here, the crystal structure of VLR39 is presented at 1.5 A resolution and compared with those of other protein-specific VLRs. VLR39 adopts a curved solenoid fold and exhibits substantial structural similarity to other protein-binding VLRs. VLR39 has a short LRRCT loop that protrudes outwards away from the concave face and is similar to those of its protein-specific VLR counterparts. Analysis of the VLR39-BCR interaction by size-exclusion chromatography and biolayer interferometry using the scFv version of the BCR confirms that VLR39 recognizes the BCR Fv region. Such VLR-based reagents may be useful for identifying and monitoring leukemia in CLL patients and in other clinical diagnostic assays.

Crystal structure of an anti-idiotype variable lymphocyte receptor.,Collins BC, Nakahara H, Acharya S, Cooper MD, Herrin BR, Wilson IA Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):682-687. doi:, 10.1107/S2053230X1701620X. Epub 2017 Nov 14. PMID:29199989<ref>PMID:29199989</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5wk4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Variable lymphocyte receptor 3D structures|Variable lymphocyte receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Petromyzon marinus]]
[[Category: Collins BC]]
[[Category: Cooper MD]]
[[Category: Herrin BR]]
[[Category: Nakahara H]]
[[Category: Wilson IA]]