View source for 6gvt


==Hybrid structure of the pRN1 helix bundle domain in complex with DNA and 2 ATP molecules==
<StructureSection load='6gvt' size='340' side='right'caption='[[6gvt]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6gvt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GVT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6GVT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Hybrid , Solid-state NMR , Solution NMR, 20 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6gvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gvt OCA], [https://pdbe.org/6gvt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6gvt RCSB], [https://www.ebi.ac.uk/pdbsum/6gvt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6gvt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q54324_SULIS Q54324_SULIS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Primases have a fundamental role in DNA replication. They synthesize a primer that is then extended by DNA polymerases. Archaeoeukaryotic primases require for synthesis a catalytic and an accessory domain, the exact contribution of the latter being unresolved. For the pRN1 archaeal primase, this domain is a 115-amino acid helix bundle domain (HBD). Our structural investigations of this small HBD by liquid- and solid-state nuclear magnetic resonance (NMR) revealed that only the HBD binds the DNA template. DNA binding becomes sequence-specific after a major allosteric change in the HBD, triggered by the binding of two nucleotide triphosphates. The spatial proximity of the two nucleotides and the DNA template in the quaternary structure of the HBD strongly suggests that this small domain brings together the substrates to prepare the first catalytic step of primer synthesis. This efficient mechanism is likely general for all archaeoeukaryotic primases.

A Small Helical Bundle Prepares Primer Synthesis by Binding Two Nucleotides that Enhance Sequence-Specific Recognition of the DNA Template.,Boudet J, Devillier JC, Wiegand T, Salmon L, Meier BH, Lipps G, Allain FH Cell. 2018 Dec 18. pii: S0092-8674(18)31556-3. doi: 10.1016/j.cell.2018.11.031. PMID:30595448<ref>PMID:30595448</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6gvt" style="background-color:#fffaf0;"></div>

==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfolobus islandicus]]
[[Category: Allain FH-T]]
[[Category: Boudet J]]
[[Category: Lipps G]]
[[Category: Meier BH]]
[[Category: Wiegand T]]