View source for 6kd0


==Crystal Structure of Vibralactone Cyclase==
<StructureSection load='6kd0' size='340' side='right'caption='[[6kd0]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kd0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Boreostereum_vibrans Boreostereum vibrans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KD0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kd0 OCA], [https://pdbe.org/6kd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kd0 RCSB], [https://www.ebi.ac.uk/pdbsum/6kd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kd0 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Vibralactone is isolated from the basidiomycete fungus Boreostereum vibrans as one of the strongest lipase inhibitors. Its unusual beta-lactone-fused bicycle is derived from an aryl ring moiety by an oxidative ring-expansion prior to an intramolecular cyclization. Herein, we report the discovery of the cyclase VibC which belongs to the alpha/beta-hydrolase superfamily and is involved in the vibralactone biosynthesis. Biochemical and crystal studies suggest that VibC may catalyze an aldol or an electrocyclic reaction initiated by the Ser-His-Asp catalytic triad. For the aldol and pericyclic chemistry in living cells, VibC is a unique hydrolase performing the carbocycle formation of an oxepinone to a fused bicyclic beta-lactone. This presents a naturally occurring, new enzymatic reaction in both aldol and hydrolase (bio)chemistry that will guide future exploitation of these enzymes in synthetic biology for chemical-diversity expansion of natural products.

A Hydrolase-Catalyzed Cyclization Forms the Fused Bicyclic beta-Lactone in Vibralactone.,Feng KN, Yang YL, Xu YX, Zhang Y, Feng T, Huang SX, Liu JK, Zeng Y Angew Chem Int Ed Engl. 2020 Apr 27;59(18):7209-7213. doi:, 10.1002/anie.202000710. Epub 2020 Mar 9. PMID:32050043<ref>PMID:32050043</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Boreostereum vibrans]]
[[Category: Large Structures]]
[[Category: Feng KN]]
[[Category: Zeng Y]]