View source for 6qlc


==The ssDNA-binding RNA polymerase cofactor Drc from Pseudomonas phage LUZ7==
<StructureSection load='6qlc' size='340' side='right'caption='[[6qlc]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6qlc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_luz7 Pseudomonas phage luz7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QLC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QLC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qlc OCA], [http://pdbe.org/6qlc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qlc RCSB], [http://www.ebi.ac.uk/pdbsum/6qlc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qlc ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Bacterial viruses encode a vast number of ORFan genes that lack similarity to any other known proteins. Here, we present a 2.20 A crystal structure of N4-related Pseudomonas virus LUZ7 ORFan gp14, and elucidate its function. We demonstrate that gp14, termed here as Drc (ssDNA-binding RNA Polymerase Cofactor), preferentially binds single-stranded DNA, yet contains a structural fold distinct from other ssDNA-binding proteins (SSBs). By comparison with other SSB folds and creation of truncation and amino acid substitution mutants, we provide the first evidence for the binding mechanism of this unique fold. From a biological perspective, Drc interacts with the phage-encoded RNA Polymerase complex (RNAPII), implying a functional role as an SSB required for the transition from early to middle gene transcription during phage infection. Similar to the coliphage N4 gp2 protein, Drc likely binds locally unwound middle promoters and recruits the phage RNA polymerase. However, unlike gp2, Drc does not seem to need an additional cofactor for promoter melting. A comparison among N4-related phage genera highlights the evolutionary diversity of SSB proteins in an otherwise conserved transcription regulation mechanism.

'Drc', a structurally novel ssDNA-binding transcription regulator of N4-related bacterial viruses.,Boon M, De Zitter E, De Smet J, Wagemans J, Voet M, Pennemann FL, Schalck T, Kuznedelov K, Severinov K, Van Meervelt L, De Maeyer M, Lavigne R Nucleic Acids Res. 2019 Nov 14. pii: 5625530. doi: 10.1093/nar/gkz1048. PMID:31724707<ref>PMID:31724707</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6qlc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas phage luz7]]
[[Category: Boon, M]]
[[Category: Lavigne, R]]
[[Category: Meervelt, L Van]]
[[Category: Smet, J De]]
[[Category: Zitter, E De]]
[[Category: Ssdna binding]]
[[Category: Viral protein]]