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==Structure of IMP bound Plasmodium falciparum IMP-nucleotidase mutant D172N==
<StructureSection load='6rme' size='340' side='right'caption='[[6rme]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RME FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IMP:INOSINIC+ACID'>IMP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rme OCA], [https://pdbe.org/6rme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rme RCSB], [https://www.ebi.ac.uk/pdbsum/6rme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rme ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Plasmodium falciparum (Pf) relies solely on the salvage pathway for its purine nucleotide requirements, making this pathway indispensable to the parasite. Purine nucleotide levels are regulated by anabolic processes and by nucleotidases that hydrolyse these metabolites into nucleosides. Certain apicomplexan parasites, including Pf, have an IMP-specific-nucleotidase 1 (ISN1). Here we show, by comprehensive substrate screening, that PfISN1 catalyzes the dephosphorylation of inosine monophosphate (IMP) and is allosterically activated by ATP. Crystal structures of tetrameric PfISN1 reveal complex rearrangements of domain organization tightly associated with catalysis. Immunofluorescence microscopy and expression of GFP-fused protein indicate cytosolic localization of PfISN1 and expression in asexual and gametocyte stages of the parasite. With earlier evidence on isn1 upregulation in female gametocytes, the structures reported in this study may contribute to initiate the design for possible transmission-blocking agents.

Structure and catalytic regulation of Plasmodium falciparum IMP specific nucleotidase.,Carrique L, Ballut L, Shukla A, Varma N, Ravi R, Violot S, Srinivasan B, Ganeshappa UT, Kulkarni S, Balaram H, Aghajari N Nat Commun. 2020 Jun 26;11(1):3228. doi: 10.1038/s41467-020-17013-x. PMID:32591529<ref>PMID:32591529</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Aghajari N]]
[[Category: Ballut L]]
[[Category: Carrique L]]
[[Category: Violot S]]