View source for 7cdx


==Complex STRUCTURE OF A NOVEL VIRULENCE REGULATION FACTOR SghR with its effector sucrose==
<StructureSection load='7cdx' size='340' side='right'caption='[[7cdx]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7cdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens_A6 Agrobacterium tumefaciens A6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CDX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.103&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cdx OCA], [https://pdbe.org/7cdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cdx RCSB], [https://www.ebi.ac.uk/pdbsum/7cdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cdx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2I4PGE9_RHIRD A0A2I4PGE9_RHIRD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Agrobacterium tumefaciens infects various plants and causes crown gall diseases involving temporal expression of virulence factors. SghA is a newly-identified virulence factor enzymatically releasing salicylic acid from its glucoside conjugate and controlling the plant tumor development. Here we report the structural basis of SghR, a LacI-type transcription factor and highly conserved in Rhizobiaceae family, regulating the expression of SghA and involved in tumorigenesis. We identified and characterized the binding site of SghR on the promoter region of sghA, and then determined the crystal structures of apo-SghR, SghR complexed with its operator DNA and ligand sucrose, respectively. These results provide detailed insights into how SghR recognizes its cognate DNA and shed a mechanistic light on how sucrose attenuates the affinity of SghR with DNA to modulate the expression of SghA. Given the important role of SghR in mediating the signaling crosstalk during Agrobacterium infection, our results pave the way for structure-based inducer analogue design, which has potential applications for agricultural industry.

Structural basis of a novel repressor SghR controlling Agrobacterium infection by cross-talking to plants.,Ye F, Wang C, Fu Q, Yan XF, Bharath SR, Casanas A, Wang M, Song H, Zhang LH, Gao YG J Biol Chem. 2020 Jul 10. pii: RA120.012908. doi: 10.1074/jbc.RA120.012908. PMID:32651231<ref>PMID:32651231</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7cdx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Agrobacterium tumefaciens A6]]
[[Category: Large Structures]]
[[Category: Gao YG]]
[[Category: Wang C]]
[[Category: Yan XF]]
[[Category: Ye FZ]]
[[Category: Zhang LH]]