View source for 7uwu


==Starch adherence system protein 6 (Sas6)==
<StructureSection load='7uwu' size='340' side='right'caption='[[7uwu]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7uwu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_bromii_L2-63 Ruminococcus bromii L2-63]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UWU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uwu OCA], [https://pdbe.org/7uwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uwu RCSB], [https://www.ebi.ac.uk/pdbsum/7uwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uwu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2N0UYM2_9FIRM A0A2N0UYM2_9FIRM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Resistant starch is a prebiotic accessed by gut bacteria with specialized amylases and starch-binding proteins. The human gut symbiont Ruminococcus bromii expresses Sas6 (Starch Adherence System member 6), which consists of two starch-specific carbohydrate-binding modules from family 26 (RbCBM26) and family 74 (RbCBM74). Here, we present the crystal structures of Sas6 and of RbCBM74 bound with a double helical dimer of maltodecaose. The RbCBM74 starch-binding groove complements the double helical alpha-glucan geometry of amylopectin, suggesting that this module selects this feature in starch granules. Isothermal titration calorimetry and native mass spectrometry demonstrate that RbCBM74 recognizes longer single and double helical alpha-glucans, while RbCBM26 binds short maltooligosaccharides. Bioinformatic analysis supports the conservation of the amylopectin-targeting platform in CBM74s from resistant-starch degrading bacteria. Our results suggest that RbCBM74 and RbCBM26 within Sas6 recognize discrete aspects of the starch granule, providing molecular insight into how this structure is accommodated by gut bacteria.

The Ruminococcus bromii amylosome protein Sas6 binds single and double helical alpha-glucan structures in starch.,Photenhauer AL, Villafuerte-Vega RC, Cerqueira FM, Armbruster KM, Marecek F, Chen T, Wawrzak Z, Hopkins JB, Vander Kooi CW, Janecek S, Ruotolo BT, Koropatkin NM Nat Struct Mol Biol. 2024 Feb;31(2):255-265. doi: 10.1038/s41594-023-01166-6. , Epub 2024 Jan 4. PMID:38177679<ref>PMID:38177679</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7uwu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ruminococcus bromii L2-63]]
[[Category: Koropatkin NM]]
[[Category: Photenhauer AL]]