View source for 7wyr


==Crystal structure of Cypovirus Polyhedra mutant fused with CLN025==
<StructureSection load='7wyr' size='340' side='right'caption='[[7wyr]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7wyr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WYR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wyr OCA], [https://pdbe.org/7wyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wyr RCSB], [https://www.ebi.ac.uk/pdbsum/7wyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wyr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYHD_CPVBM PYHD_CPVBM] Major component of the virus occlusion bodies, which are large proteinaceous structures (polyhedra), that protect the virus from the outside environment for extended periods until they are ingested by insect larvae.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protein crystals can be utilized as porous scaffolds to capture exogenous molecules. Immobilization of target proteins using protein crystals is expected to facilitate X-ray structure analysis of proteins that are difficult to be crystallized. One of the advantages of scaffold-assisted structure determination is the analysis of metastable structures that are not observed in solution. However, efforts to fix target proteins within the pores of scaffold protein crystals have been limited due to the lack of strategies to control protein-protein interactions formed in the crystals. In this study, we analyze the metastable structure of the miniprotein, CLN025, which forms a beta-hairpin structure in solution, using a polyhedra crystal (PhC), an in-cell protein crystal. CLN025 is successfully fixed within the PhC scaffold by replacing the original loop region. X-ray crystal structure analysis and molecular dynamics (MD) simulation reveal that CLN025 is fixed as a helical structure in a metastable state by non-covalent interactions in the scaffold crystal. These results indicate that modulation of intermolecular interactions can trap various protein conformations in the engineered PhC and provides a new strategy for scaffold-assisted structure determination.

Engineering of an in-cell protein crystal for fastening a metastable conformation of a target miniprotein.,Kojima M, Abe S, Furuta T, Tran DP, Hirata K, Yamashita K, Hishikawa Y, Kitao A, Ueno T Biomater Sci. 2023 Feb 14;11(4):1350-1357. doi: 10.1039/d2bm01759h. PMID:36594419<ref>PMID:36594419</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7wyr" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bombyx mori cypovirus 1]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Abe S]]
[[Category: Hirata K]]
[[Category: Kojima M]]
[[Category: Ueno T]]
[[Category: Yamashita K]]