View source for 7zy3


==Room temperature structure of Archaerhodopsin-3 obtained 110 ns after photoexcitation==
<StructureSection load='7zy3' size='340' side='right'caption='[[7zy3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7zy3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorubrum_sodomense Halorubrum sodomense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ZY3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ZY3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DD9:NONANE'>DD9</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene>, <scene name='pdbligand=R16:HEXADECANE'>R16</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7zy3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7zy3 OCA], [https://pdbe.org/7zy3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7zy3 RCSB], [https://www.ebi.ac.uk/pdbsum/7zy3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7zy3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BACR3_HALSD BACR3_HALSD] Light-driven proton pump.
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== Publication Abstract from PubMed ==
Serial crystallography has emerged as an important tool for structural studies of integral membrane proteins. The ability to collect data from micrometre-sized weakly diffracting crystals at room temperature with minimal radiation damage has opened many new opportunities in time-resolved studies and drug discovery. However, the production of integral membrane protein microcrystals in lipidic cubic phase at the desired crystal density and quantity is challenging. This paper introduces VIALS (versatile approach to high-density microcrystals in lipidic cubic phase for serial crystallography), a simple, fast and efficient method for preparing hundreds of microlitres of high-density microcrystals suitable for serial X-ray diffraction experiments at both synchrotron and free-electron laser sources. The method is also of great benefit for rational structure-based drug design as it facilitates in situ crystal soaking and rapid determination of many co-crystal structures. Using the VIALS approach, room-temperature structures are reported of (i) the archaerhodopsin-3 protein in its dark-adapted state and 110 ns photocycle intermediate, determined to 2.2 and 1.7 A, respectively, and (ii) the human A(2A) adenosine receptor in complex with two different ligands determined to a resolution of 3.5 A.

A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography.,Birch J, Kwan TOC, Judge PJ, Axford D, Aller P, Butryn A, Reis RI, Bada Juarez JF, Vinals J, Owen RL, Nango E, Tanaka R, Tono K, Joti Y, Tanaka T, Owada S, Sugahara M, Iwata S, Orville AM, Watts A, Moraes I J Appl Crystallogr. 2023 Aug 18;56(Pt 5):1361-1370. doi: , 10.1107/S1600576723006428. eCollection 2023 Oct 1. PMID:37791355<ref>PMID:37791355</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7zy3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halorubrum sodomense]]
[[Category: Large Structures]]
[[Category: Axford D]]
[[Category: Bada Juarez JF]]
[[Category: Judge PJ]]
[[Category: Kwan TOC]]
[[Category: Moraes I]]
[[Category: Watts A]]