View source for 8bc4


==Cryo-EM Structure of a BmSF-TAL - Sulfofructose Schiff Base Complex in symmetry group C1==
<StructureSection load='8bc4' size='340' side='right'caption='[[8bc4]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8bc4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_aryabhattai Priestia aryabhattai]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8BC4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8BC4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=QC9:(2~{R},3~{S},4~{S})-2,3,4,6-tetrakis(oxidanyl)hexane-1-sulfonic+acid'>QC9</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8bc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8bc4 OCA], [https://pdbe.org/8bc4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8bc4 RCSB], [https://www.ebi.ac.uk/pdbsum/8bc4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8bc4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SFTAL_PRIM1 SFTAL_PRIM1] Part of the sulfo-TAL (or sulfo-SFT) pathway, a D-sulfoquinovose degradation pathway that produces sulfolactate (SL) (PubMed:33036753). Catalyzes the conversion of 6-deoxy-6-sulfo-D-fructose (SF) and glyceraldehyde 3-phosphate (GAP) into fructose-6-phosphate (F6P) and 3-sulfolactaldehyde (SLA) (PubMed:33036753, PubMed:36805128). Can also catalyze the SF-cleavage with erythrose 4-phosphate (E4P) as acceptor, forming 3-sulfolactaldehyde (SLA) and sedoheptulose 7-phosphate (S7P) (PubMed:36805128).<ref>PMID:33036753</ref> <ref>PMID:36805128</ref> 
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== Publication Abstract from PubMed ==
Sulfoquinovose (SQ) is a key component of plant sulfolipids (sulfoquinovosyl diacylglycerols) and a major environmental reservoir of biological sulfur. Breakdown of SQ is achieved by bacteria through the pathways of sulfoglycolysis. The sulfoglycolytic sulfofructose transaldolase (sulfo-SFT) pathway is used by gut-resident firmicutes and soil saprophytes. After isomerization of SQ to sulfofructose (SF), the namesake enzyme catalyzes the transaldol reaction of SF transferring dihydroxyacetone to 3C/4C acceptors to give sulfolactaldehyde and fructose-6-phosphate or sedoheptulose-7-phosphate. We report the 3D cryo-EM structure of SF transaldolase from Bacillus megaterium in apo and ligand bound forms, revealing a decameric structure formed from two pentameric rings of the protomer. We demonstrate a covalent "Schiff base" intermediate formed by reaction of SF with Lys89 within a conserved Asp-Lys-Glu catalytic triad and defined by an Arg-Trp-Arg sulfonate recognition triad. The structural characterization of the signature enzyme of the sulfo-SFT pathway provides key insights into molecular recognition of the sulfonate group of sulfosugars.

Structure and mechanism of sulfofructose transaldolase, a key enzyme in sulfoquinovose metabolism.,Snow AJD, Sharma M, Abayakoon P, Williams SJ, Blaza JN, Davies GJ Structure. 2023 Mar 2;31(3):244-252.e4. doi: 10.1016/j.str.2023.01.010. Epub 2023 , Feb 17. PMID:36805128<ref>PMID:36805128</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8bc4" style="background-color:#fffaf0;"></div>

==See Also==
*[[Transaldolase 3D structures|Transaldolase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Priestia aryabhattai]]
[[Category: Blaza J]]
[[Category: Davies GJ]]
[[Category: Sharma M]]
[[Category: Snow AJD]]