View source for 8fss


==Complex Structure of YejA-S481A with Microcin C7==
<StructureSection load='8fss' size='340' side='right'caption='[[8fss]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8fss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FSS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7MD:5-O-[(R)-(3-AMINOPROPOXY)(L-ALPHA-ASPARTYLAMINO)PHOSPHORYL]ADENOSINE'>7MD</scene>, <scene name='pdbligand=FME:N-FORMYLMETHIONINE'>FME</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fss OCA], [https://pdbe.org/8fss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fss RCSB], [https://www.ebi.ac.uk/pdbsum/8fss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fss ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YEJA_ECOLI YEJA_ECOLI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Infections caused by gram-negative pathogens continue to be a major risk to human health because of the innate antibiotic resistance endowed by their unique cell membrane architecture. Nature has developed an elegant solution to target gram-negative strains, namely by conjugating toxic antibiotic warheads to a suitable carrier to facilitate the active import of the drug to a specific target organism. Microcin C7 (McC) is a Trojan horse peptide-conjugated antibiotic that specifically targets enterobacteria by exploiting active import through oligopeptide transport systems. Here, we characterize the molecular mechanism of McC recognition by YejA, the solute binding protein of the Escherichia coli oligopeptide transporter. Structure-guided mutational and functional analysis elucidates the determinants of substrate recognition. We demonstrate that the peptide carrier can serve as a passport for the entry of molecules that are otherwise not taken into E. coli cells. We show that peptide conjugation can remodel the antibiotic spectrum of clinically relevant parent compounds. Bioinformatics analysis reveals a broad distribution of YejA-like transporters in only the Proteobacteria, underscoring the potential for the development of Trojan horse antibiotics that are actively imported into such gram-negative bacteria.

Trojan horse peptide conjugates remodel the activity spectrum of clinical antibiotics.,Luo S, Li XR, Gong XT, Kulikovsky A, Qu F, Beis K, Severinov K, Dubiley S, Feng X, Dong SH, Nair SK Proc Natl Acad Sci U S A. 2025 Jan 7;122(1):e2319483121. doi: , 10.1073/pnas.2319483121. Epub 2024 Dec 31. PMID:39739799<ref>PMID:39739799</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8fss" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Dong S-H]]
[[Category: Nair SK]]