View source for 9nvq


==Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen and calcium==
<StructureSection load='9nvq' size='340' side='right'caption='[[9nvq]], [[Resolution|resolution]] 3.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9nvq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Halyomorpha_halys Halyomorpha halys] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NVQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene>, <scene name='pdbligand=A1B32:[(1~{S},2~{S},5~{S},6~{R},7~{S},9~{S},10~{S},18~{R})-5-cyclopropylcarbonyloxy-2,6,10-trimethyl-9,18-bis(oxidanyl)-16-oxidanylidene-14-pyridin-3-yl-11,15-dioxatetracyclo[8.8.0.0^{2,7}.0^{12,17}]octadeca-12(17),13-dien-6-yl]methyl+cyclopropanecarboxylate'>A1B32</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=D39:(2~{S})-2-azanyl-3-[[(2~{R})-3-hexadecanoyloxy-2-[(~{Z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic+acid'>D39</scene>, <scene name='pdbligand=LBN:(2R)-3-(hexadecanoyloxy)-2-{[(9Z)-octadec-9-enoyl]oxy}propyl+2-(trimethylazaniumyl)ethyl+phosphate'>LBN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nvq OCA], [https://pdbe.org/9nvq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nvq RCSB], [https://www.ebi.ac.uk/pdbsum/9nvq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nvq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A9P0HI19_NEZVI A0A9P0HI19_NEZVI] [https://www.uniprot.org/uniprot/A0A9P0MRC5_NEZVI A0A9P0MRC5_NEZVI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against underutilized targets. Nanchung (Nan) and Inactive (Iav), the transient receptor potential vanilloid-type (TRPV) channels in insects, likely form a heteromeric channel (Nan-Iav) and are localized in mechanosensory chordotonal organs which confer gravitaxis, hearing and proprioception. Several insecticides, such as afidopyropen (AP), target Nan-Iav through unknown mechanisms. Effective against piercing-sucking (hemipteran) insects, AP disrupts chordotonal functions preventing feeding. AP can bind to Nan alone, but only Nan-Iav exhibits channel activity with agonists including endogenous nicotinamide (NAM). Despite its importance as an insecticide target, much is unknown about Nan-Iav, such as channel assembly, modulator binding sites, and Ca(2+)-dependent regulation, hampering further insecticide development. Here we present the cryo-electron microscopy structures of hemipteran Nan-Iav with calmodulin bound in the apo state and with AP and NAM bound to cytosolic ankyrin repeat domain (ARD) interfaces. Unexpectedly, we found that Nan alone can form a pentamer, stabilized through AP-mediated ARD interactions. Our study provides molecular insights into insecticide and agonist interactions with Nan-Iav, highlighting the importance of the ARD on channel function and assembly, while also probing regulation by Ca(2+).

Visualizing insecticide control of insect TRP channel function and assembly.,Fedor JG, Kandasamy R, Park CG, Suo Y, Weisel M, Rankl NB, Nesterov A, Lee SY Nat Commun. 2025 Dec 14;17(1):595. doi: 10.1038/s41467-025-67287-2. PMID:41392056<ref>PMID:41392056</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9nvq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halyomorpha halys]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Fedor JG]]
[[Category: Lee S-Y]]