View source for 9smj


==Zuzalysin zymogen dodecahedral complex E439A==
<StructureSection load='9smj' size='340' side='right'caption='[[9smj]], [[Resolution|resolution]] 2.64&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9smj]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyromonas_gingivalis Porphyromonas gingivalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SMJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.64&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9smj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9smj OCA], [https://pdbe.org/9smj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9smj RCSB], [https://www.ebi.ac.uk/pdbsum/9smj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9smj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0AAF0BD41_PORGN A0AAF0BD41_PORGN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Natural pentamer dodecahedra (Ddhs) span six orders of magnitude in diameter. Among proteins, only two catalytic Ddhs have been structurally characterized: lumazine synthase (LS) and the core of pyruvate dehydrogenase (PDH). Zuzalysin (ZUZ) is a approximately 95-kDa metallopeptidase secreted for virulence by Porphyromonas gingivalis. Calcium converts latent flexible monomers into active approximately 0.5-MDa pentamers that further assemble hierarchically into bipentamers, tripentamers, and a approximately 5.6-MDa, approximately 355-A virus-like dodecahedron (Ddh(ZUZ)). Experimental structures (1.8-3.6 A) across these states reveal the molecular basis of activation, association, and catalysis, culminating in Ddh(ZUZ), which is physiologic, exceeds small viral capsids, and has 20 main entry pores and 60 lumen-facing active sites. ZUZ represents the largest catalytic protein assembly resolved at high resolution, exceeding LS, PDH, and major peptidase complexes in size and/or resolution.

Structure and Function of a Multi-Megadalton Virus-Like Proteolytic Dodecahedron.,Madej M, Rodriguez-Banqueri A, Mizgalska D, Szmigielski B, Nowakowska Z, Benedyk-Machaczka M, Bzowska M, Mikruta K, Ramirez-Larrota JS, Agunanne C, Julien O, O'Donoghue AJ, Scavenius C, Lopez-Martin M, Marcos E, Koziej L, Glatt S, Guerra P, Eckhard U, Potempa J, Gomis-Ruth FX Angew Chem Int Ed Engl. 2026 Sep 10:e5597279. doi: 10.1002/anie.5597279. PMID:42723362<ref>PMID:42723362</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9smj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Porphyromonas gingivalis]]
[[Category: Eckhard U]]
[[Category: Madej M]]
[[Category: Potempa J]]
[[Category: Rodriguez-Banqueri A]]