View source for 9vcb


==Cryo-EM structure of Shewanella putrefaciens ComEC in complex with ssDNA==
<StructureSection load='9vcb' size='340' side='right'caption='[[9vcb]], [[Resolution|resolution]] 2.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9vcb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_putrefaciens Shewanella putrefaciens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9VCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9VCB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.82&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9vcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9vcb OCA], [https://pdbe.org/9vcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9vcb RCSB], [https://www.ebi.ac.uk/pdbsum/9vcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9vcb ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Natural transformation is one of the major pathways of horizontal gene transfer in bacteria, enabling the acquisition of extracellular DNA and its integration into the host genome. ComEC is a membrane protein responsible for DNA translocation in this process, yet its precise function and structure have remained elusive. Here, we report cryo-electron microscopy structures of ComEC in DNA-free, single-stranded DNA (ssDNA)-bound, and double-stranded DNA (dsDNA)-bound forms, together with biochemical analyses. These structures reveal that ComEC cleaves one strand of dsDNA at its extracellular domain and guides the remaining strand into a positively charged pore formed within the membrane domain. These findings provide a structural basis for the long-hypothesized roles of ComEC in both DNA processing and translocation across the inner membrane during natural transformation.

Structural basis for DNA processing and membrane translocation by ComEC in natural transformation.,Hirano H, Tsuji N, Chiba S, Nureki O Science. 2026 Apr 16;392(6795):311-316. doi: 10.1126/science.aea3485. Epub 2026 , Apr 16. PMID:41990170<ref>PMID:41990170</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9vcb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Shewanella putrefaciens]]
[[Category: Synthetic construct]]
[[Category: Hirano H]]
[[Category: Nureki O]]