View source for 1waz


==NMR Structure Determination of the bacterial mercury transporter, MerF, in micelles==
<StructureSection load='1waz' size='340' side='right'caption='[[1waz]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1waz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Morganella_morganii Morganella morganii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1waz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1waz OCA], [https://pdbe.org/1waz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1waz RCSB], [https://www.ebi.ac.uk/pdbsum/1waz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1waz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q56446_MORMO Q56446_MORMO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional backbone structure of a membrane protein with two transmembrane helices in micelles was determined using solution NMR methods that rely on the measurement of backbone (1)H-(15)N residual dipolar couplings (RDCs) from samples of two different constructs that align differently in stressed polyacrylamide gels. Dipolar wave fitting to the (1)H-(15)N RDCs determines the helical boundaries based on periodicity and was utilized in the generation of supplemental dihedral restraints for the helical segments. The (1)H-(15)N RDCs and supplemental dihedral restraints enable the determination of the structure of the helix-loop-helix core domain of the mercury transport membrane protein MerF with a backbone RMSD of 0.58 A. Moreover, the fold of this polypeptide demonstrates that the two vicinal pairs of cysteine residues, shown to be involved in the transport of Hg(II) across the membrane, are exposed to the cytoplasm. This finding differs from earlier structural and mechanistic models that were based primarily on the somewhat atypical hydropathy plot for MerF and related transport proteins.

NMR structure determination of a membrane protein with two transmembrane helices in micelles: MerF of the bacterial mercury detoxification system.,Howell SC, Mesleh MF, Opella SJ Biochemistry. 2005 Apr 5;44(13):5196-206. PMID:15794657<ref>PMID:15794657</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1waz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Morganella morganii]]
[[Category: Howell SC]]
[[Category: Mesleh MF]]
[[Category: Opella SJ]]