View source for 2r75


==Aquifex aeolicus FtsZ with 8-morpholino-GTP==
<StructureSection load='2r75' size='340' side='right'caption='[[2r75]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2r75]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R75 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.402&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=01G:8-MORPHOLIN-4-YLGUANOSINE+5-(TETRAHYDROGEN+TRIPHOSPHATE)'>01G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r75 OCA], [https://pdbe.org/2r75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r75 RCSB], [https://www.ebi.ac.uk/pdbsum/2r75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r75 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FTSZ_AQUAE FTSZ_AQUAE] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r7/2r75_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r75 ConSurf].
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== Publication Abstract from PubMed ==
The cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryotic cell division and eukaryotic chromosome segregation, respectively. Selective inhibitors of the GTP-dependent polymerization of FtsZ could constitute a new class of antibiotics, while several inhibitors of tubulin are widely used in antiproliferative therapy. In this work, we set out to identify selective inhibitors of FtsZ based on the structure of its natural ligand, GTP. We found that GTP analogs with small hydrophobic substituents at C8 of the nucleobase efficiently inhibit FtsZ polymerization, whereas they have an opposite effect on the polymerization of tubulin. The inhibitory activity of the GTP analogs on FtsZ polymerization allowed us to crystallize FtsZ in complex with C8-morpholino-GTP, revealing the binding mode of a GTP derivative containing a nonmodified triphosphate chain.

Probing FtsZ and tubulin with C8-substituted GTP analogs reveals differences in their nucleotide binding sites.,Lappchen T, Pinas VA, Hartog AF, Koomen GJ, Schaffner-Barbero C, Andreu JM, Trambaiolo D, Lowe J, Juhem A, Popov AV, den Blaauwen T Chem Biol. 2008 Feb;15(2):189-99. PMID:18291323<ref>PMID:18291323</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2r75" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cell division protein 3D structures|Cell division protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aquifex aeolicus]]
[[Category: Large Structures]]
[[Category: Andreu JM]]
[[Category: Hartog AF]]
[[Category: Juhem A]]
[[Category: Koomen GJ]]
[[Category: Lappchen T]]
[[Category: Lowe J]]
[[Category: Pinas VA]]
[[Category: Popov AV]]
[[Category: Schaffner-Barbero C]]
[[Category: Trambaiolo D]]
[[Category: Den Blaauwen T]]