View source for 3ile


==Crystal structure of ORF157-E86A of Acidianus filamentous virus 1==
<StructureSection load='3ile' size='340' side='right'caption='[[3ile]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3ile]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidianus_filamentous_virus_1 Acidianus filamentous virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ILE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ILE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ile FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ile OCA], [https://pdbe.org/3ile PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ile RCSB], [https://www.ebi.ac.uk/pdbsum/3ile PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ile ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y157_AFV1Y Y157_AFV1Y] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Acidianus filamentous virus 1 (AFV1) (Lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. It infects Acidianus species that thrive in the acidic hot springs (&gt;85 degrees C and pH &lt;3) of Yellowstone National Park, WY. The AFV1 20.8-kb, linear, double-stranded DNA genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. Because three-dimensional structures are more conserved than sequences and hence are more effective at revealing function, we set out to determine protein structures from putative AFV1 open reading frames (ORF). The crystal structure of ORF157 reveals an alpha+beta protein with a novel fold that remotely resembles the nucleotidyltransferase topology. In vitro, AFV1-157 displays a nuclease activity on linear double-stranded DNA. Alanine substitution mutations demonstrated that E86 is essential to catalysis. AFV1-157 represents a novel class of nuclease, but its exact role in vivo remains to be determined.

ORF157 from the archaeal virus Acidianus filamentous virus 1 defines a new class of nuclease.,Goulet A, Pina M, Redder P, Prangishvili D, Vera L, Lichiere J, Leulliot N, van Tilbeurgh H, Ortiz-Lombardia M, Campanacci V, Cambillau C J Virol. 2010 May;84(10):5025-31. Epub 2010 Mar 3. PMID:20200253<ref>PMID:20200253</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3ile" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acidianus filamentous virus 1]]
[[Category: Large Structures]]
[[Category: Cambillau C]]
[[Category: Campanacci V]]
[[Category: Goulet A]]
[[Category: Lichiere J]]
[[Category: Prangishvili D]]
[[Category: Van Tilbeurgh H]]