View source for 4mae


==Methanol dehydrogenase from Methylacidiphilum fumariolicum SolV==
<StructureSection load='4mae' size='340' side='right'caption='[[4mae]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mae]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylacidiphilum_fumariolicum_SolV Methylacidiphilum fumariolicum SolV]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MAE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=15P:POLYETHYLENE+GLYCOL+(N=34)'>15P</scene>, <scene name='pdbligand=CE:CERIUM+(III)+ION'>CE</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mae OCA], [https://pdbe.org/4mae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mae RCSB], [https://www.ebi.ac.uk/pdbsum/4mae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mae ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XOXF_METFB XOXF_METFB] Catalyzes the oxidation of methanol to formaldehyde or formate in the presence of lanthanides (Ln). Is a key enzyme in methane/methanol metabolism, allowing M.fumariolicum to grow on methane as the sole carbon and energy source. Can also act on other primary alcohols in vitro, such as ethanol, 1-propanol, 1-butanol, and 1-hexanol, but is not able to oxidize secondary alcohols and acetaldehyde (PubMed:24034209). Uses a specific cytochrome cL, encoded by the adjacent gene in the locus, as electron acceptor (By similarity).[UniProtKB:C5B120]<ref>PMID:24034209</ref> 
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== Publication Abstract from PubMed ==
Growth of Methylacidiphilum fumariolicum SolV, an extremely acidophilic methanotrophic microbe isolated from an Italian volcanic mudpot, is shown to be strictly dependent on the presence of lanthanides, a group of rare earth elements (REEs) such as lanthanum (Ln), cerium (Ce), praseodymium (Pr) and neodymium (Nd). After fractionation of the bacterial cells and crystallization of the methanol dehydrogenase (MDH), it was shown that lanthanides were essential as cofactor in a homodimeric MDH comparable with one of the MDHs of Methylobacterium extorquens AM1. We hypothesize that the lanthanides provide superior catalytic properties to pyrroloquinoline quinone (PQQ)-dependent MDH, which is a key enzyme for both methanotrophs and methylotrophs. Thus far, all isolated MxaF-type MDHs contain calcium as a catalytic cofactor. The gene encoding the MDH of strain SolV was identified to be a xoxF-ortholog, phylogenetically closely related to mxaF. Analysis of the protein structure and alignment of amino acids showed potential REE-binding motifs in XoxF enzymes of many methylotrophs, suggesting that these may also be lanthanide-dependent MDHs. Our findings will have major environmental implications as metagenome studies showed (lanthanide-containing) XoxF-type MDH is much more prominent in nature than MxaF-type enzymes.

Rare earth metals are essential for methanotrophic life in volcanic mudpots.,Pol A, Barends TR, Dietl A, Khadem AF, Eygensteyn J, Jetten MS, Op den Camp HJ Environ Microbiol. 2013 Aug 18. doi: 10.1111/1462-2920.12249. PMID:24034209<ref>PMID:24034209</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4mae" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylacidiphilum fumariolicum SolV]]
[[Category: Barends TRM]]
[[Category: Dietl A]]
[[Category: Eygenstein J]]
[[Category: Jetten MSM]]
[[Category: Khadem AF]]
[[Category: Op den Camp HJM]]
[[Category: Pol AJ]]