View source for 4v54


==Crystal structure of the bacterial ribosome from Escherichia coli in complex with ribosome recycling factor (RRF).==
<StructureSection load='4v54' size='340' side='right'caption='[[4v54]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v54]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2qbd 2qbd], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2qbe 2qbe], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2qbf 2qbf] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2qbg 2qbg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V54 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v54 OCA], [https://pdbe.org/4v54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v54 RCSB], [https://www.ebi.ac.uk/pdbsum/4v54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v54 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL18_ECOLI RL18_ECOLI] This is one of the proteins that mediates the attachment of the 5S rRNA subcomplex onto the large ribosomal subunit where it forms part of the central protuberance. Binds stably to 5S rRNA; increases binding abilities of L5 in a cooperative fashion; both proteins together confer 23S rRNA binding. The 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.<ref>PMID:353728</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface. Aminoglycosides bind to H69 and completely restore the contacts between ribosomal subunits that are disrupted by RRF. These results provide a structural explanation for aminoglycoside inhibition of ribosome recycling.

Structural basis for aminoglycoside inhibition of bacterial ribosome recycling.,Borovinskaya MA, Pai RD, Zhang W, Schuwirth BS, Holton JM, Hirokawa G, Kaji H, Kaji A, Cate JH Nat Struct Mol Biol. 2007 Aug;14(8):727-32. Epub 2007 Jul 29. PMID:17660832<ref>PMID:17660832</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v54" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
*[[Ribosome recycling factor|Ribosome recycling factor]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Thermus thermophilus]]
[[Category: Borovinskaya MA]]
[[Category: Cate JHD]]
[[Category: Hirokawa G]]
[[Category: Holton JM]]
[[Category: Kaji A]]
[[Category: Kaji H]]
[[Category: Pai RD]]
[[Category: Schuwirth B-S]]
[[Category: Zhang W]]