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==Structure of bacterial oligosaccharyltransferase PglB in complex with an acceptor peptide and an lipid-linked oligosaccharide analog==
<StructureSection load='5ogl' size='340' side='right'caption='[[5ogl]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ogl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni] and [https://en.wikipedia.org/wiki/Campylobacter_lari_RM2100 Campylobacter lari RM2100]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OGL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9UB:[(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic+acid'>9UB</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ogl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ogl OCA], [https://pdbe.org/5ogl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ogl RCSB], [https://www.ebi.ac.uk/pdbsum/5ogl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ogl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PGLB_CAMLR PGLB_CAMLR] Oligosaccharyltransferase that catalyzes the transfer of a preassembled heptasaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains, affording a beta-linked glycan to the asparagine side chain of target proteins.<ref>PMID:21677752</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Oligosaccharyltransferase (OST) is a membrane-integral enzyme that catalyzes the transfer of glycans from lipid-linked oligosaccharides (LLOs) onto asparagine side chains, the first step in protein N-glycosylation. Here, we report the X-ray structure of a single-subunit OST, PglB from Campylobacter lari, trapped in an intermediate state bound to an acceptor peptide and a synthetic LLO analog. The structure reveals the role of the external loop EL5, present in all OST enzymes, in substrate recognition. Whereas the N-terminal half of EL5 binds LLO, the C-terminal half interacts with the acceptor peptide. The glycan moiety of LLO must thread under EL5 to access the active site. Reducing EL5 mobility decreases the catalytic rate of OST when full-size heptasaccharide LLO is provided, but not for a monosaccharide-containing LLO analog. Our results define the chemistry of a ternary complex state, assign functional roles to conserved OST motifs, and provide opportunities for glycoengineering by rational design of PglB.

Molecular basis of lipid-linked oligosaccharide recognition and processing by bacterial oligosaccharyltransferase.,Napiorkowska M, Boilevin J, Sovdat T, Darbre T, Reymond JL, Aebi M, Locher KP Nat Struct Mol Biol. 2017 Oct 23. doi: 10.1038/nsmb.3491. PMID:29058712<ref>PMID:29058712</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ogl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Campylobacter jejuni]]
[[Category: Campylobacter lari RM2100]]
[[Category: Large Structures]]
[[Category: Aebi M]]
[[Category: Boilevin J]]
[[Category: Darbre T]]
[[Category: Locher KP]]
[[Category: Napiorkowska M]]
[[Category: Reymond J-L]]
[[Category: Sovdat T]]