View source for 6rfl


==Structure of the complete Vaccinia DNA-dependent RNA polymerase complex==
<SX load='6rfl' size='340' side='right' viewer='molstar' caption='[[6rfl]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rfl]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus_GLV-1h68 Vaccinia virus GLV-1h68]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RFL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RFL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rfl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rfl OCA], [https://pdbe.org/6rfl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rfl RCSB], [https://www.ebi.ac.uk/pdbsum/6rfl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rfl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q1PIU7_9POXV Q1PIU7_9POXV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Poxviruses encode a multisubunit DNA-dependent RNA polymerase (vRNAP) that carries out viral gene expression in the host cytoplasm. We report cryo-EM structures of core and complete vRNAP enzymes from Vaccinia virus at 2.8 A resolution. The vRNAP core enzyme resembles eukaryotic RNA polymerase II (Pol II) but also reveals many virus-specific features, including the transcription factor Rap94. The complete enzyme additionally contains the transcription factor VETF, the mRNA processing factors VTF/CE and NPH-I, the viral core protein E11, and host tRNA(Gln). This complex can carry out the entire early transcription cycle. The structures show that Rap94 partially resembles the Pol II initiation factor TFIIB, that the vRNAP subunit Rpo30 resembles the Pol II elongation factor TFIIS, and that NPH-I resembles chromatin remodeling enzymes. Together with the accompanying paper (Hillen et al., 2019), these results provide the basis for unraveling the mechanisms of poxvirus transcription and RNA processing.

Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes.,Grimm C, Hillen HS, Bedenk K, Bartuli J, Neyer S, Zhang Q, Huttenhofer A, Erlacher M, Dienemann C, Schlosser A, Urlaub H, Bottcher B, Szalay AA, Cramer P, Fischer U Cell. 2019 Dec 12;179(7):1537-1550.e19. doi: 10.1016/j.cell.2019.11.024. PMID:31835032<ref>PMID:31835032</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6rfl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Vaccinia virus GLV-1h68]]
[[Category: Bartuli J]]
[[Category: Bedenk K]]
[[Category: Boettcher B]]
[[Category: Cramer P]]
[[Category: Dienemann C]]
[[Category: Erlacher M]]
[[Category: Fischer U]]
[[Category: Grimm C]]
[[Category: Hillen SH]]
[[Category: Huettenhofer A]]
[[Category: Neyer S]]
[[Category: Schlosser A]]
[[Category: Szalay AA]]
[[Category: Urlaub H]]
[[Category: Zhang Q]]