View source for 6z34


==CymD monoaromatic hydrocarbon channel==
<StructureSection load='6z34' size='340' side='right'caption='[[6z34]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6z34]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z34 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z34 OCA], [https://pdbe.org/6z34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z34 RCSB], [https://www.ebi.ac.uk/pdbsum/6z34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z34 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O33458_PSEPU O33458_PSEPU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In modern societies, biodegradation of hydrophobic pollutants generated by industry is important for environmental and human health. In Gram-negative bacteria, biodegradation depends on facilitated diffusion of the pollutant substrates into the cell, mediated by specialised outer membrane (OM) channels. Here we show, via a combined experimental and computational approach, that the uptake of monoaromatic hydrocarbons such as toluene in Pseudomonas putida F1 (PpF1) occurs via lateral diffusion through FadL channels. Contrary to classical diffusion channels via which polar substrates move directly into the periplasmic space, PpF1 TodX and CymD direct their hydrophobic substrates into the OM via a lateral opening in the channel wall, bypassing the polar barrier formed by the lipopolysaccharide leaflet on the cell surface. Our study suggests that lateral diffusion of hydrophobic molecules is the modus operandi of all FadL channels, with potential implications for diverse areas such as biodegradation, quorum sensing and gut biology.

Uptake of monoaromatic hydrocarbons during biodegradation by FadL channel-mediated lateral diffusion.,Somboon K, Doble A, Bulmer D, Basle A, Khalid S, van den Berg B Nat Commun. 2020 Dec 10;11(1):6331. doi: 10.1038/s41467-020-20126-y. PMID:33303757<ref>PMID:33303757</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6z34" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas putida]]
[[Category: Van den Berg B]]