View source for 7kh3


==Crystal structure of the MarR family transcriptional regulator from Variovorax paradoxus bound to Indole 3 propionic acid==
<StructureSection load='7kh3' size='340' side='right'caption='[[7kh3]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7kh3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Variovorax_paradoxus Variovorax paradoxus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7KH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7KH3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOP:INDOLYLPROPIONIC+ACID'>IOP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7kh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7kh3 OCA], [https://pdbe.org/7kh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7kh3 RCSB], [https://www.ebi.ac.uk/pdbsum/7kh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7kh3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C5CSP2_VARPS C5CSP2_VARPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Chemical signalling in the plant microbiome can have drastic effects on microbial community structure, and on host growth and development. Previously, we demonstrated that the auxin metabolic signal interference performed by the bacterial genus Variovorax via an auxin degradation locus was essential for maintaining stereotypic root development in an ecologically relevant bacterial synthetic community. Here, we dissect the Variovorax auxin degradation locus to define the genes iadDE as necessary and sufficient for indole-3-acetic acid (IAA) degradation and signal interference. We determine the crystal structures and binding properties of the operon's MarR-family repressor with IAA and other auxins. Auxin degradation operons were identified across the bacterial tree of life and we define two distinct types on the basis of gene content and metabolic products: iac-like and iad-like. The structures of MarRs from representatives of each auxin degradation operon type establish that each has distinct IAA-binding pockets. Comparison of representative IAA-degrading strains from diverse bacterial genera colonizing Arabidopsis plants show that while all degrade IAA, only strains containing iad-like auxin-degrading operons interfere with auxin signalling in a complex synthetic community context. This suggests that iad-like operon-containing bacterial strains, including Variovorax species, play a key ecological role in modulating auxins in the plant microbiome.

Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.,Conway JM, Walton WG, Salas-Gonzalez I, Law TF, Lindberg CA, Crook LE, Kosina SM, Fitzpatrick CR, Lietzan AD, Northen TR, Jones CD, Finkel OM, Redinbo MR, Dangl JL Nat Microbiol. 2022 Nov;7(11):1817-1833. doi: 10.1038/s41564-022-01244-3. Epub , 2022 Oct 20. PMID:36266335<ref>PMID:36266335</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7kh3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Variovorax paradoxus]]
[[Category: Dangl JL]]
[[Category: Redinbo MR]]
[[Category: Walton WG]]