View source for 7ncx


==Crystal structure of GH30 (double mutant EE) from Thermothelomyces thermophila.==
<StructureSection load='7ncx' size='340' side='right'caption='[[7ncx]], [[Resolution|resolution]] 1.36&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7ncx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus_ATCC_42464 Thermothelomyces thermophilus ATCC 42464]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NCX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ncx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ncx OCA], [https://pdbe.org/7ncx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ncx RCSB], [https://www.ebi.ac.uk/pdbsum/7ncx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ncx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/XY30A_MYCTT XY30A_MYCTT] Xylanase exhibiting endo- and exo-xylanase activity (PubMed:31110561). Shows the highest activity toward beechwood glucuronoxylan, which consists of a beta-1,4-linked xylose backbone decorated with the methylated form of D-glucuronic acid (MeGlcA) attached directly to the main chain at xylose C2 (PubMed:31110561). Acts also against wheat arabinoxylan, a xylan without MeGlcA substituents along the main chain, but the xylanase activity is about two orders of magnitude lower than that achieved in the case of beechwood xylan (PubMed:31110561). Shows no activity against carob galactomannan, konjac glucomannan, or barley beta-glucan (PubMed:31110561). The recombinant xylanase also exhibits an exo-activity by releasing processively disaccharide units from the non-reducing end of linear and decorated xylooligosaccharides (XOS) (PubMed:31110561).<ref>PMID:31110561</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermothelomyces thermophilus ATCC 42464]]
[[Category: Dimarogona M]]
[[Category: Feiler CG]]
[[Category: Nikolaivits E]]
[[Category: Topakas E]]
[[Category: Weiss M]]