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==Crystal Structure of the B subunit of heat labile enterotoxin LT-IIc from Escherichia coli in complex with Sialyl-lacto-N-neotetraose d==
<StructureSection load='7prs' size='340' side='right'caption='[[7prs]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PRS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7prs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7prs OCA], [https://pdbe.org/7prs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7prs RCSB], [https://www.ebi.ac.uk/pdbsum/7prs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7prs ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The heat-labile enterotoxins of Escherichia coli and cholera toxin of Vibrio cholerae are related in structure and function. Each of these oligomeric toxins are comprised of one A polypeptide and five B polypeptides. The B-subunits bind to gangliosides, which is followed by uptake into the intoxicated cell and activation of the host's adenylate cyclase by the A-subunits. There are two antigenically distinct groups of these toxins. Group I includes cholera toxin and type I heat-labile enterotoxin of E. coli; group II contains the type II heat-labile enterotoxins of E. coli. Three variants of type II toxins, designated LT-IIa, LT-IIb and LT-IIc have been described. Earlier studies revealed the crystalline structure of LT-IIb. Herein the carbohydrate binding specificity of LT-IIc B-subunits was investigated by glycosphingolipid binding studies on thin-layer chromatograms and in microtiter wells. Binding studies using a large variety of glycosphingolipids showed that LT-IIc binds with high affinity to gangliosides with a terminal Neu5Ac&lt;3Gal or Neu5Gc&lt;3Gal sequence, e.g. the gangliosides GM3, GD1a and Neu5Ac&lt;3-/Neu5Gc&lt;3-neolactotetraosylceramide and Neu5Ac&lt;3-/Neu5Gc&lt;3-neolactohexaosylceramide. The crystal structure of LT-IIc B-subunits alone and with bound LSTd/sialyl-lacto-N-neotetraose d pentasaccharide uncovered the molecular basis of the ganglioside recognition. These studies revealed common and unique functional structures of the type II family of heat-labile enterotoxins.

Characterization of the ganglioside recognition profile of Escherichia coli heat-labile enterotoxin LT-IIc.,Zalem D, Juhas M, Terrinoni M, King-Lyons N, Lebens M, Varrot A, Connell TD, Teneberg S Glycobiology. 2021 Dec 24. pii: 6482028. doi: 10.1093/glycob/cwab133. PMID:34972864<ref>PMID:34972864</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Varrot A]]