View source for 7qyf


==Structure of the transaminase PluriZyme variant (TR2E2)==
<StructureSection load='7qyf' size='340' side='right'caption='[[7qyf]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7qyf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidihalobacter Acidihalobacter]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QYF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qyf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qyf OCA], [https://pdbe.org/7qyf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qyf RCSB], [https://www.ebi.ac.uk/pdbsum/7qyf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qyf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3G5BC54_9GAMM A0A3G5BC54_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Engineering dual-function single polypeptide catalysts with two abiotic or biotic catalytic entities (or combinations of both) supporting cascade reactions is becoming an important area of enzyme engineering and catalysis. Herein we present the development of a PluriZyme, TR(2) E(2) , with efficient native transaminase (k(cat) : 69.49+/-1.77 min(-1) ) and artificial esterase (k(cat) : 3908-0.41 min(-1) ) activities integrated into a single scaffold, and evaluate its utility in a cascade reaction. TR(2) E(2) (pH(opt) : 8.0-9.5; T(opt) : 60-65 degrees C) efficiently converts methyl 3-oxo-4-(2,4,5-trifluorophenyl)butanoate into 3-(R)-amino-4-(2,4,5-trifluorophenyl)butanoic acid, a crucial intermediate for the synthesis of antidiabetic drugs. The reaction proceeds through the conversion of the beta-keto ester into the beta-keto acid at the hydrolytic site and subsequently into the beta-amino acid (e.e. &gt;99 %) at the transaminase site. The catalytic power of the TR(2) E(2) PluriZyme was proven with a set of beta-keto esters, demonstrating the potential of such designs to address bioinspired cascade reactions.

A Plurizyme with Transaminase and Hydrolase Activity Catalyzes Cascade Reactions.,Roda S, Fernandez-Lopez L, Benedens M, Bollinger A, Thies S, Schumacher J, Coscolin C, Kazemi M, Santiago G, Gertzen CGW, Gonzalez-Alfonso JL, Plou FJ, Jaeger KE, Smits SHJ, Ferrer M, Guallar V Angew Chem Int Ed Engl. 2022 Sep 12;61(37):e202207344. doi: , 10.1002/anie.202207344. Epub 2022 Aug 4. PMID:35734849<ref>PMID:35734849</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qyf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Acidihalobacter]]
[[Category: Large Structures]]
[[Category: Benedens M]]
[[Category: Bollinger A]]
[[Category: Coscolin C]]
[[Category: Fernandez-Lopez L]]
[[Category: Ferrer M]]
[[Category: Gertzen CG]]
[[Category: Gonzalez-Alfonso J]]
[[Category: Guallar V]]
[[Category: Jaeger KE]]
[[Category: Kazemi M]]
[[Category: Plou FJ]]
[[Category: Roda S]]
[[Category: Santiago G]]
[[Category: Schumacher J]]
[[Category: Smits SH]]
[[Category: Thies S]]