View source for 7uqa


==Crystal structure of the small Ultra-Red Fluorescent Protein (smURFP)==
<StructureSection load='7uqa' size='340' side='right'caption='[[7uqa]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7uqa]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UQA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.802&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uqa OCA], [https://pdbe.org/7uqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uqa RCSB], [https://www.ebi.ac.uk/pdbsum/7uqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uqa ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The small Ultra-Red Fluorescent Protein (smURFP) represents a new class of fluorescent protein with exceptional photostability and brightness derived from allophycocyanin in a previous directed evolution. Here, we report the smURFP crystal structure to better understand properties and enable further engineering of improved variants. We compare this structure to the structures of allophycocyanin and smURFP mutants to identify the structural origins of the molecular brightness. We then use a structure-guided approach to develop monomeric smURFP variants that fluoresce with phycocyanobilin but not biliverdin. Furthermore, we measure smURFP photophysical properties necessary for advanced imaging modalities, such as those relevant for two-photon, fluorescence lifetime, and single-molecule imaging. We observe that smURFP has the largest two-photon cross-section measured for a fluorescent protein, and that it produces more photons than organic dyes. Altogether, this study expands our understanding of the smURFP, which will inform future engineering toward optimal FPs compatible with whole organism studies.

Structural and photophysical characterization of the small ultra-red fluorescent protein.,Maiti A, Buffalo CZ, Saurabh S, Montecinos-Franjola F, Hachey JS, Conlon WJ, Tran GN, Hassan B, Walters KJ, Drobizhev M, Moerner WE, Ghosh P, Matsuo H, Tsien RY, Lin JY, Rodriguez EA Nat Commun. 2023 Jul 12;14(1):4155. doi: 10.1038/s41467-023-39776-9. PMID:37438348<ref>PMID:37438348</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7uqa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Buffalo CZ]]
[[Category: Conlon WJ]]
[[Category: Drobizhev M]]
[[Category: Ghosh P]]
[[Category: Hachey JS]]
[[Category: Lin JY]]
[[Category: Maiti A]]
[[Category: Matsuo H]]
[[Category: Moerner WE]]
[[Category: Montecinos-Franjola F]]
[[Category: Rodriguez EA]]
[[Category: Saurabh S]]
[[Category: Tran GN]]
[[Category: Tsien RY]]