View source for 7yjw


==Structure of Leptospira santarosai serovar shermani LRR protein LSS01692==
<StructureSection load='7yjw' size='340' side='right'caption='[[7yjw]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[7yjw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_santarosai_serovar_Shermani_str._LT_821 Leptospira santarosai serovar Shermani str. LT 821]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7YJW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7YJW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7yjw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7yjw OCA], [https://pdbe.org/7yjw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7yjw RCSB], [https://www.ebi.ac.uk/pdbsum/7yjw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7yjw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K8Y546_9LEPT K8Y546_9LEPT] 
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== Publication Abstract from PubMed ==
Leptospirosis is a commonly overlooked zoonotic disease that occurs in tropical and subtropical regions. Recent studies have divided the Leptospira spp. into three groups based on virulence, including pathogenic, intermediate, and saprophytic species. Pathogenic species express a protein family with leucine-rich repeat (LRR) domains, which are less expressed or absent in nonpathogenic species, highlighting the importance of this protein family in leptospirosis. However, the role of LRR domain proteins in the pathogenesis of leptospirosis is still unknown and requires further investigation. In this study, the 3D structure of LSS_01692 (rLRR38) was obtained using X-ray crystallography at a resolution of 3.2 A. The results showed that rLRR38 forms a typical horseshoe structure with 11 alpha-helices and 11 beta-sheets and an antiparallel dimeric structure. The interactions of rLRR38 with extracellular matrix and cell surface receptors were evaluated using ELISA and single-molecule atomic force microscopy. The results showed that rLRR38 interacted with fibronectin, collagen IV, and Toll-like receptor 2 (TLR2). Incubating HK2 cells with rLRR38 induced two downstream inflammation responses (IL-6 and MCP-1) in the TLR2 signal transduction pathway. The TLR2-TLR1 complex showed the most significant upregulation effects under rLRR38 treatment. Inhibitors also significantly inhibited nuclear factor kappaB and mitogen-activated protein kinases signals transduction under rLRR38 stimulation. In conclusion, rLRR38 was determined to be a novel LRR domain protein in 3D structure and demonstrated as a TLR2-binding protein that induces inflammatory responses. These structural and functional studies provide a deeper understanding of the pathogenesis of leptospirosis.

Crystal structure of Leptospira LSS_01692 reveals a dimeric structure and induces inflammatory responses through Toll-like receptor 2-dependent NF-kappaB and MAPK signal transduction pathways.,Hsu SH, Wu CT, Sun YJ, Chang MY, Li C, Ko YC, Chou LF, Yang CW FEBS J. 2023 May 27. doi: 10.1111/febs.16874. PMID:37243454<ref>PMID:37243454</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7yjw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Leptospira santarosai serovar Shermani str. LT 821]]
[[Category: Hsu SH]]
[[Category: Sun YJ]]
[[Category: Wu CT]]
[[Category: Yang CW]]