View source for 8fwg


==Structure of neck and portal vertex of Agrobacterium phage Milano, C5 symmetry==
<StructureSection load='8fwg' size='340' side='right'caption='[[8fwg]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8fwg]] is a 165 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_phage_Milano Agrobacterium phage Milano]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FWG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.45&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fwg OCA], [https://pdbe.org/8fwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fwg RCSB], [https://www.ebi.ac.uk/pdbsum/8fwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fwg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A482MFR0_9CAUD A0A482MFR0_9CAUD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.

Neck and capsid architecture of the robust Agrobacterium phage Milano.,Sonani RR, Esteves NC, Horton AA, Kelly RJ, Sebastian AL, Wang F, Kreutzberger MAB, Leiman PG, Scharf BE, Egelman EH Commun Biol. 2023 Sep 8;6(1):921. doi: 10.1038/s42003-023-05292-1. PMID:37684529<ref>PMID:37684529</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8fwg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Agrobacterium phage Milano]]
[[Category: Large Structures]]
[[Category: Egelman EH]]
[[Category: Esteves NC]]
[[Category: Kelly RJ]]
[[Category: Kreutzberger MAB]]
[[Category: Leiman PG]]
[[Category: Scharf BE]]
[[Category: Sebastian A]]
[[Category: Sonani RR]]
[[Category: Wang F]]