View source for 8hiw


==AtALMT9 in the apo state==
<StructureSection load='8hiw' size='340' side='right'caption='[[8hiw]], [[Resolution|resolution]] 3.34&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8hiw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HIW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.34&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hiw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hiw OCA], [https://pdbe.org/8hiw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hiw RCSB], [https://www.ebi.ac.uk/pdbsum/8hiw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hiw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ALMT9_ARATH ALMT9_ARATH] Vacuolar malate channel. Has a higher selectivity for malate than for fumarate. Exhibits also a weak chloride conductance.<ref>PMID:18005230</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Arabidopsis thaliana aluminum-activated malate transporter 9 (AtALMT9) functions as a vacuolar chloride channel that regulates the stomatal aperture. Here, we present the cryoelectron microscopy (cryo-EM) structures of AtALMT9 in three distinct states. AtALMT9 forms a dimer, and the pore is lined with four positively charged rings. The apo-AtALMT9 state shows a putative endogenous citrate obstructing the pore, where two W120 constriction residues enclose a gate with a pore radius of approximately 1.8 A, representing an open state. Interestingly, channel closure is solely controlled by W120. Compared to wild-type plants, the W120A mutant exhibits more sensitivity to drought stress and is unable to restore the visual phenotype on leaves upon water recovery, reflecting persistent stomatal opening. Furthermore, notable variations are noted in channel gating and substrate recognition of Glycine max ALMT12, AtALMT9, and AtALMT1. In summary, our investigation enhances comprehension of the interplay between structure and function within the ALMT family.

Structural insight into the Arabidopsis vacuolar anion channel ALMT9 shows clade specificity.,Qian D, Chai Y, Li W, Cui B, Lin S, Wang Z, Wang C, Qu LQ, Gong D Cell Rep. 2024 Sep 12;43(9):114731. doi: 10.1016/j.celrep.2024.114731. PMID:39269901<ref>PMID:39269901</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 8hiw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Gong DS]]