View source for 9fyv


==Crystal structure of the engineered photoenzyme VEnT1.3==
<StructureSection load='9fyv' size='340' side='right'caption='[[9fyv]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9fyv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Loligo_vulgaris Loligo vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9FYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9FYV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1IHG:(2~{S})-2-azanyl-3-(9-oxidanylidenethioxanthen-2-yl)propanoic+acid'>A1IHG</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9fyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9fyv OCA], [https://pdbe.org/9fyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9fyv RCSB], [https://www.ebi.ac.uk/pdbsum/9fyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9fyv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DFPA_LOLVU DFPA_LOLVU] Biological function and substrate unknown. However, it is capable of acting on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including nerve gases).<ref>PMID:15966726</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The development of [2 + 2] cyclases containing benzophenone triplet sensitizers highlights the potential of engineered enzymes as a platform for stereocontrolled energy transfer photocatalysis. However, the suboptimal photophysical features of benzophenone necessitates the use of ultraviolet light, limits photochemical efficiency and restricts the range of chemistries accessible. Here we engineer an orthogonal Methanococcus jannaschii tyrosyl-tRNA synthetase/tRNA pair for encoding thioxanthone triplet sensitizers into proteins, which can efficiently harness visible light to drive photochemical conversions. Initially, we developed an enantioselective [2 + 2] cyclase that is orders of magnitude more efficient than our previously developed photoenzymes (k(cat) = 13 s(-1), &gt;1,300 turnovers). To demonstrate that thioxanthone-containing enzymes can enable more challenging photochemical conversions, we developed a second oxygen-tolerant enzyme that can steer selective C-H insertions of excited quinolone substrates to afford spirocyclic beta-lactams with high selectivity (99% e.e., 22:1 d.r.). This photoenzyme also suppresses a competing substrate decomposition pathway observed with small-molecule sensitizers, underscoring the ability of engineered enzymes to control the fate of excited-state intermediates.

Efficient and selective energy transfer photoenzymes powered by visible light.,Crawshaw R, Smithson R, Hofer J, Hardy FJ, Roberts GW, Trimble JS, Kohn AR, Levy CW, Drost DA, Merten C, Heyes DJ, Obexer R, Bach T, Green AP Nat Chem. 2025 May 6. doi: 10.1038/s41557-025-01820-0. PMID:40329013<ref>PMID:40329013</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9fyv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Loligo vulgaris]]
[[Category: Hardy FJ]]
[[Category: Roberts GW]]