View source for 9qm4


==Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis (alternative N-terminus)==
<StructureSection load='9qm4' size='340' side='right'caption='[[9qm4]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9qm4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_bottropensis Streptomyces bottropensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9QM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9QM4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9qm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9qm4 OCA], [https://pdbe.org/9qm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9qm4 RCSB], [https://www.ebi.ac.uk/pdbsum/9qm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9qm4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/W8QLE8_9ACTN W8QLE8_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aromatic polyketides from Actinobacteria are structurally complex bioactive natural products with significant therapeutic potential, whose biosynthesis involves polyketide chain assembly, keto reduction, cyclization, and aromatization. This is followed by pathway-specific enzymatic tailoring steps, occasionally including rare oxidative rearrangements of the carbon skeleton, as exemplified by the rishirilides. In this study, we investigate RslO9, a flavin-dependent tailoring key enzyme of rishirilide biosynthesis, previously hypothesized to facilitate a lactone-forming Baeyer-Villiger oxidation of the rishirilide naphthoquinone core and subsequent intramolecular aldol condensation. Through detailed investigation of RslO9's mechanism, structural features, and substrate scope, we unexpectedly found that the naphthoquinone moiety of the non-natural substrate lapachol undergoes hydroxylation followed by a benzilic acid rearrangement, producing the Hooker intermediate-a hallmark of the intricate Hooker oxidation. Our data support a similar alkyl migration mechanism for RslO9's native substrate, upending its prior classification as a Baeyer-Villiger monooxygenase and challenging the proposed role of related enzymes while also providing a novel framework for exploring their catalytic roles.

A Hooker Oxygenase Archetype in Polyketide Biosynthesis Challenging the Baeyer-Villiger Monooxygenase Paradigm.,Weddeling HG, Sowa ST, Bialas E, Reese S, Merten C, Lill M, Bechthold A, Teufel R J Am Chem Soc. 2026 Jan 26. doi: 10.1021/jacs.5c21759. PMID:41588612<ref>PMID:41588612</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9qm4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces bottropensis]]
[[Category: Sowa ST]]
[[Category: Teufel R]]
[[Category: Weddeling HG]]