View source for 2bls

[[Image:2bls.gif|left|200px]]<br />
<applet load="2bls" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2bls, resolution 2.0&Aring;" />
'''AMPC BETA-LACTAMASE FROM ESCHERICHIA COLI'''<br />

==Overview==
The structures of AmpC beta-lactamase from Escherichia coli, alone and in, complex with a transition-state analogue, have been determined by X-ray, crystallography. The native enzyme was determined to 2.0 A resolution, and, the structure with the transition-state analogue m-aminophenylboronic acid, was determined to 2.3 A resolution. The structure of AmpC from E. coli, resembles those previously determined for the class C enzymes from, Enterobacter cloacae and Citrobacter freundii. The transition-state, analogue, m-aminophenylboronic acid, makes several interactions with AmpC, that were unexpected. Perhaps most surprisingly, the putative "oxyanion", of the boronic acid forms what appears to be a hydrogen bond with the, backbone carbonyl oxygen of Ala318, suggesting that this atom is, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9819201 (full description)]]

==About this Structure==
2BLS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]]. Active as [[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Structure known Active Sites: ACB and ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLS OCA]]. 

==Reference==
Three-dimensional structure of AmpC beta-lactamase from Escherichia coli bound to a transition-state analogue: possible implications for the oxyanion hypothesis and for inhibitor design., Usher KC, Blaszczak LC, Weston GS, Shoichet BK, Remington SJ, Biochemistry. 1998 Nov 17;37(46):16082-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9819201 9819201]
[[Category: Beta-lactamase]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Blaszczak, L.C.]]
[[Category: Remington, S.J.]]
[[Category: Usher, K.C.]]
[[Category: Wery, J.P.]]
[[Category: beta-lactamase]]
[[Category: cephalosporinase]]
[[Category: serine hydrolase]]

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