View source for 2b08

[[Image:2b08.gif|left|200px]]<br /><applet load="2b08" size="350" color="white" frame="true" align="right" spinBox="true" 
caption="2b08, resolution 1.90&Aring;" />
'''Reduced acetamide-bound M150G Nitrite Reductase from Alcaligenes faecalis'''<br />

==Overview==
Copper-containing nitrite reductase harbors a type-1 and a type-2 Cu site. The former acts as the electron acceptor site of the enzyme, and the latter is the site of catalytic action. The effect of the methionine ligand on the reorganization energy of the type-1 site was explored by studying the electron-transfer kinetics between NiR (wild type (wt) and the variants Met150Gly and Met150Thr) with Fe(II)EDTA and Fe(II)HEDTA. The mutations increased the reorganization energy by 0.3 eV (30 kJ mol-1). A similar increase was found from pulse radiolysis experiments on the wt NIR and three variants (Met150Gly, Met150His, and Met150Thr). Binding of the nearby Met62 to the type-1 Cu site in Met150Gly (under influence of an allosteric effector) lowered the reorganization energy back to approximately the wt value. According to XRD data the structure of the reduced type-1 site in Met150Gly NiR in the presence of an allosteric effector is similar to that in the reduced wt NiR (solved to 1.85 A), compatible with the similarity in reorganization energy.

==About this Structure==
2B08 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=CU1:'>CU1</scene> and <scene name='pdbligand=ACM:'>ACM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B08 OCA]. 

==Reference==
Effect of the methionine ligand on the reorganization energy of the type-1 copper site of nitrite reductase., Wijma HJ, MacPherson I, Farver O, Tocheva EI, Pecht I, Verbeet MP, Murphy ME, Canters GW, J Am Chem Soc. 2007 Jan 24;129(3):519-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17227014 17227014]
[[Category: Alcaligenes faecalis]]
[[Category: Nitrite reductase (NO-forming)]]
[[Category: Single protein]]
[[Category: Canters, G W.]]
[[Category: Farver, O.]]
[[Category: MacPherson, I S.]]
[[Category: Murphy, M E.P.]]
[[Category: Pecht, I.]]
[[Category: Tocheva, E I.]]
[[Category: Verbeet, M Ph.]]
[[Category: Wijma, H J.]]
[[Category: ACM]]
[[Category: CU1]]
[[Category: acetamide]]
[[Category: allosteric control]]
[[Category: axial methionine]]
[[Category: met62]]
[[Category: reorganization energy]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:32:50 2008''