View source for 1ecc

[[Image:1ecc.gif|left|200px]]<br />
<applet load="1ecc" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="1ecc, resolution 2.4&Aring;" />
'''ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH MN-CPRPP AND 5-OXO-NORLEUCINE'''<br />

==Overview==
Activation of gluatmine phosphoribosylpyrophosphate (RPPP), amidotransferase (GPATase) by binding of a PRPP substrate analog results, in the formation of a 20 A channel connecting the active site for, glutamine hydrolysis in one domain with the PRPP site in a second domain., This solvent-inaccessible channel permits transfer of the NH3 intermediate, between the two active sites. Tunneling of NH3 may be a common mechanism, for glutamine amidotransferase-catalyzed nitrogen transfer and for, coordination of catalysis at two distinct active sites in complex enzymes., The 2.4 A crystal structure of the active conformer of GPATase also, provides the first description of an intact active site for the, phosphoribosyltransferase (PRTase) family of nucleotide synthesis and, salvage enzymes. ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9333323 (full description)]]

==About this Structure==
1ECC is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with MN, ONL and PCP as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ECC OCA]]. 

==Reference==
Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site., Krahn JM, Kim JH, Burns MR, Parry RJ, Zalkin H, Smith JL, Biochemistry. 1997 Sep 16;36(37):11061-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9333323 9333323]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Krahn, J.M.]]
[[Category: Smith, J.L.]]
[[Category: MN]]
[[Category: ONL]]
[[Category: PCP]]
[[Category: glutamine amidotransferase]]
[[Category: glycosyltransferase]]
[[Category: purine biosynthesis]]
[[Category: transferase]]

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