View source for 2bh4

[[Image:2bh4.gif|left|200px]]<br />
<applet load="2bh4" size="450" color="white" frame="true" align="right" spinBox="true" 
caption="2bh4, resolution 1.55&Aring;" />
'''X-RAY STRUCTURE OF THE M100K VARIANT OF FERRIC CYT C-550 FROM PARACOCCUS VERSUTUS DETERMINED AT 100 K.'''<br />

==Overview==
The structure of cytochrome c-550 from the nonphotosynthetic bacteria, Paraccocus versutus has been solved by X-ray crystallography to 1.90 A, resolution, and reveals a high structural homology to other bacterial, cytochromes c(2). The effect of replacing the axial heme-iron methionine, ligand with a lysine residue on protein structure and unfolding has been, assessed using the M100K variant. From X-ray structures at 1.95 and 1.55 A, resolution it became clear that the amino group of the lysine side chain, coordinates to the heme-iron. Structural differences compared to the, wild-type protein are confined to the lysine ligand loop connecting, helices four and five. In the heme cavity an additional water molecule is, found which participates in an H-bonding interaction with the lysine, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15885094 (full description)]]

==About this Structure==
2BH4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paracoccus_versutus Paracoccus versutus]] with HEC as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BH4 OCA]]. 

==Reference==
The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c-550 from Paracoccus versutus assessed by X-ray crystallography and unfolding., Worrall JA, van Roon AM, Ubbink M, Canters GW, FEBS J. 2005 May;272(10):2441-55. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15885094 15885094]
[[Category: Paracoccus versutus]]
[[Category: Single protein]]
[[Category: Canters, G.W.]]
[[Category: Roon, A.M.M.Van.]]
[[Category: Ubbink, M.]]
[[Category: Worrall, J.A.R.]]
[[Category: HEC]]
[[Category: axial ligand]]
[[Category: c-type cytochrome]]
[[Category: electron transfer]]
[[Category: heme]]
[[Category: pyrrolidone carboxylic acid]]

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:37:36 2007''